高等学校化学学报 ›› 1992, Vol. 13 ›› Issue (11): 1386.

• 研究简报 • 上一篇    下一篇

毛细管凝胶电泳的激光光热在线检测

邓延倬, 于沛, 叶梦炜, 刘恒彪   

  1. 武汉大学分析测试科学系, 武汉, 430072
  • 收稿日期:1991-11-12 修回日期:1992-08-21 出版日期:1992-11-24 发布日期:1992-11-24
  • 通讯作者: 邓延倬

Laser Opto-thermal On-line Detection for the Gel Capillary Electrophoresis

DENG Yan-Zhuo, YU Pei, YE Meng-Wei, LIU Heng-Biao   

  1. Department of Analysis and Testing Science, Wuhan University, Wuhan, 430072
  • Received:1991-11-12 Revised:1992-08-21 Online:1992-11-24 Published:1992-11-24

关键词: 激光, 光热检测, 毛细管电泳

Abstract: The opto-thermal detection technique based on crossed-beam thermal lens effect has been developed for the on-line determination of capillary polyacrylamide gel electrophoresis(PAGE).The minimum detectable concentration of methylene blue in ethanol filled into 220 pm i.d.capillary was obtained to be 1.76×10-9 mol/L,corresponding to the absorbance of 3.8×10-6.Amixture of bromophenol blue and amide black was separated by capillary PAGE and detected by laser opto-thermal on-line detection technique.Mass detection limit for bromophenol blue injected into the gel capillary was found to be 40 pg.

Key words: Laser, Opto-thertnal detection, Capillary electrophoresis

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