高等学校化学学报 ›› 2013, Vol. 34 ›› Issue (7): 1565-1570.doi: 10.7503/cjcu20130148

• 研究论文: 无机化学 • 上一篇    下一篇

聚甲基丙烯酰胺包覆的ZnO发光量子点及其在细胞成像中的应用

徐玉东1, 张正勇1, 孔继烈1, 李国栋2, 熊焕明1   

  1. 1. 复旦大学化学系, 上海 200433;
    2. 无机合成与制备化学国家重点实验室, 吉林大学化学学院, 长春 130012
  • 收稿日期:2013-02-15 出版日期:2013-07-10 发布日期:2013-06-21
  • 通讯作者: 熊焕明, 男, 博士, 教授, 博士生导师, 主要从事功能型聚合物纳米杂化材料的研究. E-mail: hmxiong@fudan.edu.cn E-mail:hmxiong@fudan.edu.cn
  • 基金资助:

    国家重大科学研究计划项目(批准号: 2013CB934102);国家自然科学基金(批准号: 21271045)和吉林大学无机合成与制备化学国家重点实验室开放课题(批准号: 2013-13)资助.

Photoluminescent ZnO Quantum Dots Coated by Polymethacrylamide and Their Application in Cell Imaging

XU Yu-Dong1, ZHANG Zheng-Yong1, KONG Ji-Lie1, LI Guo-Dong2, XIONG Huan-Ming1   

  1. 1. Department of Chemistry, Fudan University, Shanghai 200433, China;
    2. State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012, China
  • Received:2013-02-15 Online:2013-07-10 Published:2013-06-21

摘要:

通过溶胶-凝胶法合成了在水溶液中稳定发光、荧光颜色可调的ZnO@polymer核壳型纳米粒子, 并研究了这种量子点对人类宫颈癌HeLa细胞的毒性以及细胞吞噬后激光共聚焦成像的效果. 这种荧光探针的外壳是一种通过配位键与ZnO内核结合的共聚物, 该共聚物外层是亲水的聚甲基丙烯酰胺, 内层是疏水的聚甲基丙烯酸酯. 细胞毒性实验证明, 该材料有良好的生物兼容性, 适用于生物荧光标记. 共聚焦荧光成像结果显示, 这些纳米粒子可以穿透HeLa细胞膜并在细胞质中稳定发光.

关键词: ZnO量子点, 光致发光, 表面修饰, 细胞毒性, 激光共聚焦成像

Abstract:

ZnO@polymer core-shell nanoparticles with stable luminescence in aqueous solutions and tunable emission were synthesized by sol-gel method, studied in cytotoxicity tests towards HeLa cells and measured by laser confocal scanning microscope. The shell of such luminescent probes is a copolymer connected the ZnO core through coordination bonds, which contains an external hydrophilic layer of polymethacrylamide and an internal hydrophobic layer of polymethacrylate. Such design protected the ZnO cores from decomposition by aqueous solutions on one hand, and assured the highly dispersity and long-term stability in water on the other hand. Cytotoxicity measurements proved this material is biocompatible and suitable for biological labels. The results of the laser confocal imaging showed these nanoparticles could penetrate the membranes of HeLa cells and exhibited stable fluorescence in the cytoplasm.

Key words: ZnO quantum dots, Photoluminescence, Surface modification, Cytotoxicity, Laser confocal imaging

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