高等学校化学学报 ›› 2016, Vol. 37 ›› Issue (7): 1276-1281.doi: 10.7503/cjcu20150980

• 分析化学 • 上一篇    下一篇

市售保健品中7种醒酒护肝功效成分的毛细管电泳测定

邹海民1,2, 周琛1, 孙成均1, 李永新1, 杨晓松2, 文君2, 曾红燕1()   

  1. 1. 四川大学华西公共卫生学院, 成都 610041
    2. 成都市疾病预防控制中心, 成都 610047
  • 收稿日期:2015-12-23 出版日期:2016-07-10 发布日期:2016-06-02
  • 基金资助:
    科技部“十二五”科技支撑计划(批准号: 2012BAD33B02)资助

Simultaneous Determination of 7 Components in Functional Food for Anti-hangover and Hepatoprotection by Capillary Electrophoresis

ZOU Haimin1,2, ZHOU Chen1, SUN Chengjun1, LI Yongxin1, YANG Xiaosong2, WEN Jun2, ZENG Hongyan1,*()   

  1. 1. West China School of Public Health, Sichuan University, Chengdu 610041, China
    2. Chengdu Center for Disease Control & Prevention, Chengdu 610047, China
  • Received:2015-12-23 Online:2016-07-10 Published:2016-06-02
  • Contact: ZENG Hongyan E-mail:zhm504532@163.com

摘要:

采用胶束电动毛细管电泳-二极管阵列检测器同时检测了市售保健品中儿茶素、 表儿茶素、 表没食子儿茶素、 表儿茶素没食子酸酯、 表没食子儿茶素没食子酸酯、 二氢杨梅素和甘草酸等7种醒酒护肝功效成分. 采用正交设计法对毛细管电泳条件[缓冲剂浓度、 添加剂十二烷基硫酸钠(SDS)浓度、 添加剂乙腈比例以及电泳缓冲溶液pH]进行了优化. 在优化的条件下, 7种组分在各自的线性范围内相关系数r≥0.9989, 检出限和定量限分别为0.26~2.22 μg/g(S/N=3)和0.87~7.39 μg/g(S/N=10), 日内精密度和日间精密度分别为1.3%~2.5%和1.9%~3.9%, 样品加标回收率为91.4%~104.9%, 加标样品的相对标准偏差(RSD)在1.4%~3.2%之间. 本方法可在8 min内实现7种功效成分的同时检测, 能够满足市售醒酒护肝产品的常规分析和质量评价要求.

关键词: 胶束电动毛细管电泳, 正交设计, 儿茶素, 二氢杨梅素, 甘草酸

Abstract:

A rapid method of micellar electrokinetic capillary electrophoresis(CE) coupled with diode array detection was developed for simultaneous determination of catechin, epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate, dihydromyricetin and glycyrrhizic acid in functional food for anti-hangover and hepatoprotection. The parameters of capillary electrophoresis, including buffer concentration, concentration of sodium dodecyl sulfate(SDS), volume ratio of acetonitrile, and pH of the buffer, were optimized with orthogonal design. Under the optimal analytical conditions, the peak area of each analyte and its concentration had a good correlation within the linear range(r≥0.9989). Limit of detection(LOD) and quantification(LOQ) of the method were in the range of 0.26—2.22 μg/g(S/N=3) and 0.87—7.39 μg/g(S/N=10), respectively. The intra- and inter-day relative standard deviations(RSDs) of the mixed standard solution were 1.3%—2.5% and 1.9%—3.9%, respectively. While the spiked recoveries of the analytes were 91.4%—104.9% and the RSDs of the spiked samples were 1.4%—3.2%. The method of capillary electrophoresis for determination of the 7 components in functional food for anti-hangover and hepatoprotection was proposed in this study and could achieve baseline separation for all the target components within 8 min. The results show that the method could meet the requirement for routine analysis and quality control and evaluation.

Key words: Micellar electrokinetic capillary electrophoresis, Orthogonal design, Catechin, Dihydromyricetin, Glycyrrhizic acid(Ed.: D, K)

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