高等学校化学学报 ›› 2014, Vol. 35 ›› Issue (7): 1409-1413.doi: 10.7503/cjcu20140109

• 分析化学 • 上一篇    下一篇

生物组织中丝氨酸对映体的毛细管电泳-非接触式电导分离检测

韦昱, 陈裕富, 周强, 袁秋月, 谭凤玉, 谢天尧()   

  1. 中山大学化学与化学工程学院, 广州 510275
  • 收稿日期:2014-02-14 出版日期:2014-07-10 发布日期:2014-05-19
  • 作者简介:联系人简介: 谢天尧, 男, 博士, 副教授, 主要从事毛细管电泳分析研究. E-mail: cesxty@mail.sysu.edu.cn
  • 基金资助:
    国家自然科学基金委员会人才培养基金(批准号: J1103305)和广东省大学生创新计划项目基金(批准号: G1055812202)资助

Enantioseparation of Underivatized D,L-Serine in Biological Matrices by Capillary Electrophoresis with Contactless Conductivity Detection

WEI Yu, CHEN Yufu, ZHOU Qiang, YUAN Qiuyue, TAN Fengyu, XIE Tianyao*()   

  1. School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275
  • Received:2014-02-14 Online:2014-07-10 Published:2014-05-19
  • Contact: XIE Tianyao E-mail:cesxty@mail.sysu.edu.cn
  • Supported by:
    Supported by the National Natural Science Foundation of China(NoJ1103305) and the Fund of Innovative Chemical Experiment and Research for Undergraduate Students of Guangdong Province, China(NoG1055812202)

摘要:

采用高效毛细管电泳-非接触电导检测法, 使用未涂层石英毛细管(50 μm i.d.×45 cm, Leff=40 cm), 以3.2 mmol/L氢氧化钠+0.4 mmol/L柠檬酸+2.5 mmol/L乙酸铜+5.0 mmol/L L-精氨酸+15.0 mg/L 羟丙基甲基纤维素为电泳运行液, 实现了未衍生化的D,L-丝氨酸对映体的基线分离和检测. 其线性范围为0.35~30 mg/L, 检出限(S/N=3)为0.10 mg/L. 对影响分离度的因素如手性选择剂、 电泳运行液的组成和浓度、 分离电压和进样方式等进行了探讨. 结果表明, 该方法无需衍生化预处理, 高效低耗, 可实现常见氨基酸共存组分的不干扰测定, 能有效测定小鼠脑组织样品中的D-丝氨酸含量.

关键词: D-丝氨酸, 对映体分离, 毛细管电泳, 非接触式电导检测, 生物组织样品

Abstract:

A new method for enantioseparation of underivatized D,L-serine in biological matrices by capillary electrophoresis with contactless conductivity detection using binary chiral selectors(Cu-L-Arg and HPMC) was developed.The enantiomers of D,L-serine could reach an excellent baseline separation in an uncoated fused-silica capillary(50 μm i.d.×45 cm, Leff=40 cm) under the chosen separation conditions and using 3.2 mmol/L NaOH+0.4 mmol/L Cit+2.5 mmol/L Cu(Ac)2+5.0 mmol/L L-Arg +15.0 mg/L HPMC(pH=9.8)as the running buffer. The calibration curve of the enantiomers showed good linearity in the range of 0.35—30 mg/L with a limit of detection(LOD) of 0.10 mg/L. Effects of several factors on resolutions, such as the composition of the running buffer, pH, separation voltage and injection method were investigated to acquire the optimum conditions. The proposed method, without derivatization, has been applied to determine the D-serine contents in rat brain tissue, and was demonstrated simple, efficient, cost-effective and reproducible.

Key words: D-Serine, Enantioseparation, Capillary electrophoresis, Contactless conductivity detection, Biological matrices sample(Ed.: N, K)

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