高等学校化学学报

高等学校化学学报 ›› 1991, Vol. 12 ›› Issue (6): 847.

• 论文 • 上一篇    下一篇

用桑蚕丝素蛋白固定的葡萄糖氧化酶传感器

邵正中, 方跃, 于同隐, 邓家祺   

  1. 复旦大学材料科学系、化学系, 上海, 200433
  • 收稿日期:1990-12-28 出版日期:1991-06-24 发布日期:1991-06-24
  • 通讯作者: 于同隐
  • 基金资助:

    国家自然科学基金

A Sensor of Glucose Oxidase Immobilized in Bombyx Mori Silk Fibroin Membrane

Shao Zheng-zhong, Fang Yue, Yu Tong-yin, Deng Jia-qi   

  1. Department of Material Science and Departmment of Chemistry, Fudan University, Shanghai, 200433
  • Received:1990-12-28 Online:1991-06-24 Published:1991-06-24

PDF (PC)

被引次数

7

摘要： 目前,常用的生物酶固定化方法在应用上各有优劣,简便、高效而又适用推广的酶固定化方法是人们所追求的目标。本文提供一种新型的酶固定化方法,即通过蚕丝素蛋白在甲醇中变性,使葡萄糖氧化酶(GOD)固定在蛋白质中,得到葡萄糖氧化酶-丝素膜,与氧电极偶合,制成电流式葡萄糖传感器。在选定的实验条件下,电极的各项性能指标均较满意。GOD由BoehringerMannhenGmbH进口(10kU/46mg),试剂均为分析纯。

关键词: 丝素蛋白, 酶, 固定化, 传感器

Abstract: Glucose oxidase (GOD) can be immobilized in Bombyx mori silk fibroin membrane. This is due to the structural transition of the silk fibroin membrane from random coil to antiparalled β-sheet induced by methanol treatment. Glucose sensor was made of this GOD-immobilized fibroin membrane. The calibration curve, response time, recovery, thermal and storage stability were determined.

Key words: Fibroin, Enzyme, Immobilization, Sensor

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