高等学校化学学报 ›› 2020, Vol. 41 ›› Issue (7): 1513-1520.doi: 10.7503/cjcu20200133

• 分析化学 • 上一篇    下一篇

氮硫共掺杂碳量子点对胃液pH值的精确检测

黄加玲1,刘凤娇1,2,王婷婷1,刘翠娥1,郑凤英1,2,王振红1,李顺兴1,2,*()   

  1. 1.闽南师范大学化学化工与环境学院, 漳州 363000
    2.现代分离分析科学与技术福建省重点实验室&污染监测与控制福建省高校重点实验室, 漳州 363000
  • 收稿日期:2020-03-12 出版日期:2020-07-10 发布日期:2020-05-20
  • 通讯作者: 李顺兴 E-mail:lishunxing@mnnu.edu.cn
  • 基金资助:
    国家自然科学基金(21675077);福建省高校产学合作项目(2019Y4010);福建省高等学校新世纪优秀人才支持计划

Nitrogen and Sulfur co-Doped Carbon Quantum Dots for Accurate Detection of pH in Gastric Juice

HUANG Jialing1,LIU Fengjiao1,2,WANG Tingting1,LIU Cuie1,ZHENG Fengying1,2,WANG Zhenhong1,LI Shunxing1,2,*()   

  1. 1. College of Chemistry, Chemical Engineering and Environment, Minnan Normal University, Zhangzhou 363000, China
    2. Fujian Province Key Laboratory of Modern Analytical Science and Separation Technology & Pollution Monitoring and Control, Minnan Normal University, Zhangzhou 363000, China
  • Received:2020-03-12 Online:2020-07-10 Published:2020-05-20
  • Contact: Shunxing LI E-mail:lishunxing@mnnu.edu.cn
  • Supported by:
    † National Natural Science Foundation of China(21675077);Project of Industry-University-Research Cooperation of Fujian Province, China(2019Y4010);Program for New Century Excellent Talents in Fujian Province University, China

摘要:

以水杨酸和硫脲为原料, 通过水热法一步合成水溶性好、 稳定性强、 量子产率高(36.23%)、 呈蓝光的氮硫共掺杂碳量子点(N,S-CQDs). 在302 nm波长激发下, N,S-CQDs在发射波长409 nm处的荧光强度与pH值(在2.01~5.11范围内)线性关系良好, 相关系数为0.9931, 可原位、 实时检测胃液内pH值在Δ0.01范围内的变化. 可用于胃癌早期诊断和新生儿健康防护. 400 μg/mL N,S-CQDs孵育铜绿微囊藻细胞48 h, 存活率82.8%, 其可作为人体胃细胞荧光探针, 经人体排泄进入水环境, 对淡水浮游生物毒性低, 环境友好.

关键词: 碳量子点, 掺杂, pH值, 胃液, 荧光探针

Abstract:

Nitrogen-sulfur co-doped carbon quantum dots with blue light, good water solubility, strong stability and high quantum yield(36.23%), were synthesized by one-step hydrothermal method, using salicylic acid and thiourea as raw materials. Under the excitation of 302 nm, there is a good linear relationship between the fluorescence intensity and the pH value in the range of 2.01—5.11 at the emission wavelength of 409 nm, and the correlation coefficient is 0.9931. The change of pH value in gastric juice can be detected in situ and in real time. In view of the fact that the pH value of gastric juice is closely related to the diagnosis of gastric cancer and neonatal monitoring, N,S-CQDs are expected to contribute to the early diagnosis of gastric cancer and neonatal health protection. N,S-CQDs have low cytotoxicity and good biocompatibility. When microcystis aeruginosa cells as phytoplankton model are incubated with 400 μg/mL of N,S-CQDs for 48 h, their survival ratesare 82.8%, indicating that N,S-CQDs could be used as a environmental-friendly fluorescent probe for human gastric cells.

Key words: Carbon quantum dots, Doping, pH value, Gastric juice, Fluorescent probe

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