高等学校化学学报 ›› 2014, Vol. 35 ›› Issue (11): 2366.doi: 10.7503/cjcu20140201

• 有机化学 • 上一篇    下一篇

荧光自旋捕获探针的合成及ESR捕获研究

王兵1, 杜立波3, 刘阳平2, 周建威1(), 刘扬3()   

  1. 1. 贵州师范大学化学与材料科学学院, 贵阳550001
    2. 天津医科大学药学院, 天津 300070
    3. 中国科学院化学研究所分子动态与稳态结构国家重点实验室, 北京 100190
  • 收稿日期:2014-03-11 出版日期:2014-11-10 发布日期:2014-10-09
  • 作者简介:联系人简介: 周建威, 男, 博士, 教授, 主要从事磁共振波谱研究. E-mail:jianweizhou_2000@yahoo.com;刘 扬, 男, 博士, 研究员, 主要从事自由基生物学研究. E-mail:yliu@iccas.ac.cn
  • 基金资助:
    国家自然科学基金(批准号: 31300697, 81201126, 21063004)资助

Synthesis of a Fluorescence Labeled Spin Trap and Its ESR Study

WANG Bing1, DU Libo3, LIU Yangping2, ZHOU Jianwei1,*(), LIU Yang3,*()   

  1. 1. School of Chemistry and Materials Science, Guizhou Normal University, Guiyang 550001, China
    2. School of Pharmacy, Tianjin Medical University, Tianjin 300070, China
    3. State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Center for Molecular Science,Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
  • Received:2014-03-11 Online:2014-11-10 Published:2014-10-09
  • Contact: ZHOU Jianwei,LIU Yang E-mail:jianweizhou_2000@yahoo.com;yliu@iccas.ac.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos.31300697, 81201126, 21063004)

摘要:

设计合成了具有荧光基团的新型硝酮类自由基捕获探针并对其结构进行了表征. 自由基捕获实验结果表明, 该探针能实现对超氧阴离子自由基与碳中心自由基的捕获. 此外, 该自由基捕获探针反应产物的荧光强度与被捕获自由基浓度之间存在相关性, 有望建立依据荧光强度分析被捕获自由基浓度的新方法.

关键词: 自由基, 硝酮, 自旋捕获, 荧光, 超氧阴离子

Abstract:

Free radicals play a key role in physiological and pathological processes including aging and cancer development. To understand the biological role of free radicals, it is of critical importance to develop highly sensitive techniques for the detection and quantitation of free radicals being produced. Among the numbers of techniques used for the detection of free radicals, electron spin resonance(ESR)-spin trapping technique, owing to its relative higher specificity, is believed to be an indispensable method for the detection of free radicals in biological systems. However, the ESR-spin trapping technique was limited becauseit has the relative low sensitivity and it’s hard to achieve real-time detection of free radicals in living cells. Considering that the fluorescence probes for labeling and tracking subcellular structures in living cells are easily available, a new spin trap conjugated with fluorescence moiety(DanPPN) was synthesized and characterized. The ESR study showed that the DanPPN could be used for the detection of superoxide anion, oxygen and carbon-centered radicals. Moreover, it was observed that the fluorescence intensity of DanPPN was decreased with the increase of the concentration of AIBN. The results suggested that the new spin trap may have a potential use for the detection of free radicals in biological systems.

Key words: Free radical, Nitrone, Spin trap, Fluorescence, Superoxide anion

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