高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (12): 2638.doi: 10.7503/cjcu20120404

• 分析化学 • 上一篇    下一篇

重金属离子富集检测的微量热研究

王丽萍1, 胥义1, 郑艺华2   

  1. 1. 上海理工大学生物系统热科学研究所, 上海 200093;
    2. 青岛大学机电工程学院, 生物系统热科学研究所, 青岛 266071
  • 收稿日期:2012-04-26 出版日期:2012-12-10 发布日期:2012-11-20
  • 通讯作者: 胥义,男,博士,副教授,主要从事生物系统热科学技术研究.E-mail:xu_hongyi@263.net E-mail:xu_hongyi@263.net
  • 基金资助:

    国家自然科学基金(批准号: 50906056, 50806036)、 上海市教育委员会创新项目(批准号: 11YZ111)和上海市科学技术委员会启明星项目(批注号: 08QA14052)资助.

Heavy Metal Ions Enrichment Detection with Micro-calorimetry

WANG Li-Ping1, XU Yi1, ZHENG Yi-Hua2   

  1. 1. Institute of Biothermal Science & Technology, University of Shanghai for Science & Technology, Shanghai 200093, China;
    2. Institute of Biothermal Science & Technology, College of Mechanical Engineering, Qingdao University, Qingdao 266071, China
  • Received:2012-04-26 Online:2012-12-10 Published:2012-11-20

摘要:

采用微量热技术对重金属离子的富集检测过程进行了研究. 采用流动注射分析法研究了重金属离子进样顺序、 样品进样流速和载体树脂颗粒粒径对重金属离子抑制脲酶催化反应的影响, 优化了脲酶及重金属离子进样量, 获得了脲酶固定化及重金属离子富集条件的优化参数. 结果表明, 在低流速(0.1 mL/min), 载体树脂颗粒粒径为0.5~0.6 mm, 脲酶进样量为3 mL, 重金属离子进样量为5 mL的条件下, 先进行重金属离子的富集, 再检测其对固定化脲酶催化反应的影响可得到较好的检测效果. 在实验浓度范围内, 4种重金属离子对脲酶催化反应的影响顺序为砷离子>铜离子>镉离子>铅离子. 本文对重金属离子进行了适当的富集, 降低其检测下限, 为采用微量热法进行重金属离子的快速检测提供了理论依据.

关键词: 微量热法, 富集, 酶抑制法, 重金属离子, 抑制率

Abstract:

Enrichment effects of heavy metal ions on urease-catalyzed reaction were investigated with micro-calorimetry. The effects of heavy metal ions inject order, flow rates(0.1, 0.5 and 1 mL/min) and micro-particle diameters(0.5-0.6, 0.7-0.8 and 0.9-1.0 mm) on urease-catalyzed reactions inhibited by heavy metals were investigated experimentally based on flow injection analysis(FIA) method. Furtherly, some optimal conditions such as inject volumes of urease and heavy metal ions were also obtained. The results showed that a better thermal reaction signal could be detected for a low flow rate of 0.1 mL/min, micro-particle diameters of 0.5—0.6 mm, and urease and heavy metals inject volume are 3 and 5 mL, respectively. The inhibition effects as following: As3+>Cu2+> Cd2+> Pb2+. These results indicate that the micro-calorimetry may be a potential method to rapidly and accurately detect heavy metals when the detected heavy metal ions are enrichment appropriately.

Key words: Micro-calorimetry, Enrichment, Enzyme inhibition, Heavy metal ion, Inhibition rate

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