高等学校化学学报 ›› 2019, Vol. 40 ›› Issue (6): 1135.doi: 10.7503/cjcu20180799

• 分析化学 • 上一篇    下一篇

膜富集结合膜上图像比色法快速检测微量有机磷农药

熊芩1, 杨吴烨1, 杨少杰2, 刘静3, 杨式华3, 陈万超1, 李龙1, 杜一平1()   

  1. 1. 华东理工大学化学与分子工程学院, 上海市功能性材料化学重点实验室, 上海 200237
    2. 云南省科学技术院, 昆明 650228
    3. 云南同创检测技术股份有限公司, 昆明 650106
  • 收稿日期:2018-11-27 出版日期:2019-06-10 发布日期:2019-05-11
  • 作者简介:

    联系人简介: 杜一平, 男, 博士, 教授, 博士生导师, 主要从事分子光谱及化学计量学的研究. E-mail: yipingdu@ecust.edu.cn

  • 基金资助:
    云南省科技计划项目(2016RA015)资助.

Rapid Detection of Trace Organophosphorus Pesticides by Membrane Enrichment Combined with Membrane Image Colorimetric

XIONG Qin1, YANG Wuye1, YANG Shaojie2, LIU Jing2, YANG Shihua3, CHEN Wanchao1, LI Long1, DU Yiping1()   

  1. 1. School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai Key Laboratory of Functional Materials Chemistry, Shanghai 200237, China
    2. Yunnan Provincial Academy of Science and Technology, Kunming 650228, China
    3. Yunnan Comtestor Co., Ltd., Kunming 650106, China
  • Received:2018-11-27 Online:2019-06-10 Published:2019-05-11
  • Supported by:
    † Sopported by the Science and Technology Project of Yunnan Province, China(No.2016RA015).

摘要:

建立了基于手机图像比色法结合多通道膜富集技术用于微量有机磷农药的快速检测方法. 根据乙酰胆碱酯酶活性受有机磷农药特异性抑制而降低的特点, 以吲哚乙酸酯为底物, 经酶水解生成靛蓝, 利用多通道膜富集装置将有色生成物富集到尼龙微孔滤膜上, 将标准溶液和样品溶液富集在同一张膜的不同位置. 用智能手机拍摄微孔滤膜的数码照片, 利用编制的手机APP软件对照片进行处理, 消除光照、 噪点等的影响, 可实现自动识别标准和未知样品. 通过提取样品颜色信号, 建立了颜色信号与浓度的定量分析模型, 用其检测敌百虫的检出限可达0.030 mg/L. 利用该方法对黄瓜中农药敌百虫的含量进行分析, 加标回收率在95.8%~106.9%之间, 相对标准偏差RSD(n=5)为3.8%~5.4%. 本方法用手机代替光谱仪, 简单实用, 与相关国标方法相比, 灵敏度和准确度均得到显著提高, 具有良好的应用前景.

关键词: 智能手机, 膜富集, 图像比色法, 敌百虫

Abstract:

A new method was developed for the rapid detection of organophosphorus pesticides based on smartphone and multi-channel membrane enrichment. According to the property that the activity of acetylcholinesterase was reduced by the specific inhibition of organophosphorus pesticides, indole acetate was used as a substrate which can be hydrolyzed by enzyme to form indigo. Indigo was enriched on a nylon membrane by the multi-channel membrane enrichment device with which analyte in standard solutions and sample solutions can be enriched on the same membrane at different positions. A mobile APP was developed to detect the membrane. Digital photographs of membrane were collected by the phone. With the APP, the influence of illumination and noise was eliminated and all samples enriched at different positions on the membrane could be identified automatically, the color signal of the samples were extracted, thus a standard curve of the color signal vs. concentration of the pesticide could be regressed. Limit of detection of trichlorfon was estimated being 0.030 mg/L. The content of trichlorfon in cucumber was analyzed with the developed method. The results showed that the recoveries were 95.8%—106.9% with relative standard deviations of 3.8%—5.4%. Using a smartphone but spectrometer, the proposed method is simple and applicable. Comparing with the method of GB/T 5009.100-2003, sensitivity and accuracy are improved significantly. The method showed potential applications in fast detection fields.

Key words: Smartphone, Membrane enrichment, Image colorimetric, Trichlorphon

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