高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (07): 1493-1497.doi: 10.3969/j.issn.0251-0790.2012.07.024

• 生物化学 • 上一篇    下一篇

磷酸化对D1蛋白7900和9300 降解片段的影响

陈良兵1,2, 杜立波1, 田秋1, 贾宏瑛1, 高艳丽1,2, 刘扬1   

  1. 1. 中国科学院化学研究所分子动态与稳态结构国家重点实验室, 北京 100190;
    2. 中国科学院研究生院, 北京 100049
  • 收稿日期:2011-10-12 出版日期:2012-07-10 发布日期:2012-07-10
  • 通讯作者: 刘 扬, 男, 博士, 研究员, 博士生导师, 主要从事生物自由基的研究. E-mail: yliu@iccas.ac.cn E-mail:yliu@iccas.ac.cn
  • 基金资助:

    国家自然科学基金(批准号: 20875093, 90813021)资助.

Effect of Phosphorylation on the Production of 7900 and 9300 Fragments of D1 Protein

CHEN Liang-Bing1,2, DU Li-Bo1, TIAN Qiu1, JIA Hong-Ying1, GAO Yan-Li1,2, LIU Yang1   

  1. 1. State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China;
    2. Graduate University of the Chinese Academy of Sciences, Beijing 100049, China
  • Received:2011-10-12 Online:2012-07-10 Published:2012-07-10

摘要: 利用Western blotting技术对未磷酸化和磷酸化光合系统Ⅱ(PSⅡ)膜中D1蛋白的7900和9300降解片段进行了检测. 结果表明, 超氧阴离子、 过氧化氢和羟基自由基参与了未磷酸化和磷酸化D1蛋白的7900和9300片段的产生. 磷酸化能够部分抑制D1蛋白7900和9300片段的产生, 从而对D1蛋白起到保护作用.

关键词: D1蛋白, 磷酸化, 电子自旋共振, 自由基, 光合系统Ⅱ

Abstract: So far it is unclear whether the phosphorylation protects D1 protein by decreasing the production of 7900 and 9300 fragments of this protein, and reactive oxygen species affect the production of these two fragments in phosphorylation condition. In this study, 7900 and 9300 fragments of D1 protein were detected in non-phosphorylated and phosphorylated photosystem Ⅱ(PSⅡ) membranes using Western blotting technique. It was found that reactive oxygen species including superoxide anion, hydrogen peroxide and hydroxyl radical were involved in the production of 7900 and 9300 fragments of D1 protein in both the non-phosphorylated and phosphorylated forms. And it was also found that phosphorylation could partially inhibit the production of 7900 and 9300 fragments of D1 protein, which played a protective role in D1 protein.

Key words: D1 protein, Phosphorylation, Electron spin resonance(ESR), Free radical, Photosystem Ⅱ

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