Kinetics of Catalytic Hydrolysis of Phosphodiester and Plasmid DNA by the Quaternary Ammonium Salt of Chitosan with Zn(Ⅱ)

Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (12): 2828.

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Kinetics of Catalytic Hydrolysis of Phosphodiester and Plasmid DNA by the Quaternary Ammonium Salt of Chitosan with Zn(Ⅱ)

PENG Ying-Gui¹, ZHANG Qi², XIANG Yan^1,2*

1. School of Chemistry and Environment,
2. School of Materials Science and Engineering, Beihang University, Beijing 100191, China

Received:2011-03-22 Revised:2011-05-04 Online:2011-12-10 Published:2011-11-25
Contact: XIANG Yan E-mail:xiangy@buaa.edu.cn
Supported by:
国家自然科学基金(批准号: 21073010)资助.

Abstract

Abstract: The hydroxypropyl trimethyl ammonium chloride chitosan(HTACC) was synthesized by the reaction of chitosan with glycidyl trimethylammonium chloride. The kinetics of hydrolysis of bis(p-nitrophenyl)phosphate(BNPP) catalyzed by HTACC-Zn(Ⅱ) in Tris-H+ buffer was studied and analyzed. In addition, the cleavage of plasmid DNA and the molecular interaction with HTACC-Zn(Ⅱ) were also investigated. The results show that the catalyst has fine catalytic activity to the hydrolysis of phosphodiesters with a first-order rate constant of kobs＝6.7×10-6 s-1, which is 6.0×10-4 folds of its spontaneous hydrolysis. Furthermore, the catalyst can cleavage plasmid DNA(pUC19) from supercoiled form to nicked form and/or linear form effectively.

Key words: Chitosan, Quaternary ammonium salt, Phosphodiester, Catalytic hydrolysis, DNA cleavage

CLC Number:

O643

TrendMD:

PENG Ying-Gui, ZHANG Qi, XIANG Yan*. Kinetics of Catalytic Hydrolysis of Phosphodiester and Plasmid DNA by the Quaternary Ammonium Salt of Chitosan with Zn(Ⅱ)[J]. Chem. J. Chinese Universities, 2011, 32(12): 2828.

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Kinetics of Catalytic Hydrolysis of Phosphodiester and Plasmid DNA by the Quaternary Ammonium Salt of Chitosan with Zn(Ⅱ)

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