Application of Surface-enhanced Raman Spectroscopy in the Fast Screening of Phosphodiesterase Type 5 Inhibitors†

doi:10.7503/cjcu20160710

Abstract

Abstract: Aim

ing at the absence of a fast and effective screening technique facing the prevalence of the illegal adulterant of Phosphodiesterase type 5(PDE-5) inhibitor in health products, a non-designated detection platform based on surface-enhanced Raman spectroscopy(SERS) was proposed. Integrated with a simple pretreatment process in 3—5 min, SERS measurement was carried out on those suspicious health product samples with the lowest detectable concentration at a level of 0.05 mg/kg. The advantages of short time and high sensitivity satisfy the urgent demand of the fast screening and on-site inspection of the authorities.

Key words: Surface-enhanced Raman spectroscopy, Phosphodiesterase type 5(PDE-5), Non-targeting quick screening, High sensitivity

CLC Number:

O657.37

TrendMD:

WANG Hongmei, LI Lingling, CHEN Haibin, ZENG Yongming, CHEN Hongju, CHEN Qizhen, LIN Huizhen, TIAN Zhongqun, LIU Guokun. Application of Surface-enhanced Raman Spectroscopy in the Fast Screening of Phosphodiesterase Type 5 Inhibitors^†[J]. Chem. J. Chinese Universities, 2017, 38(6): 1040.

Figures/Tables 4

Fig.1 SERS spectra of 1 mg/kg sildenafil group(A), acetildenafil group(B), vardenafil group(C), tadalafil group(D) and thioaildenafil(E) and SERS spectra of sildenafil, acetildenafil, vardenafil, tadalafil and thioaildenafil for comparison(F)

Fig.2 Molecular structures of 11 PDE-5 inhibitors

Fig.3 Sildenafil concentration dependent normalized SERS intensity of the 1234 cm-1 peak(A) and the corresponding SERS spectra(B)Concentration of sildenafil/(mg·kg-1): a. 0; b. 0.05; c. 0.1; d. 5; e. 10.

Fig.4 SERS spectra of commercial certain brand of Tadalafil(a), powder of Hippocampus(b), Sildenafil(c), Platium Kidney(d) and Platinum Testosterone(e) after the same pretreatment procedure

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