Label-free Colorimetric Assay Based on Gold Nanoparticles in Detection of NOS1AP with Single Nucleotide Polymorphism

Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (10): 1965.

• Articles • Previous Articles Next Articles

Label-free Colorimetric Assay Based on Gold Nanoparticles in Detection of NOS1AP with Single Nucleotide Polymorphism

GUO Qing-Chuan1,2, WANG Xiang1, LOU Xin-Hui1, MAO Hong-Ju1*, JIA Jie3*, JIN Qing-Hui1, ZHAO Jian-Long1, ZHANG Feng3

1. Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China;
2. Department of Rehabilitation, Third Hospital, Xinxiang Medical College, Xinxiang 453003, China;
3. Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China

Received:2009-12-30 Online:2010-10-10 Published:2010-10-10
Contact: MAO Hong-Ju, E-mail: hjmao@mail.sim.ac.cn; JIA Jie, E-mail: drjiajie@163.com
Supported by:
国家重大科学计划项目(批准号: 2009ZX10004-105)和上海市科委纳米专项基金(批准号: 0952nm05700)资助.

Abstract

Abstract: A gold nanoparticles’ colorimetry-based assay for detection of NOS1AP gene with single nucleotide polymorphism was established, which profited from the dissimilar electrostatic interactions between single-stranded oligonucleotides and double-stranded oligonucleotides. The detection probes, the oligonucleotides with their perfectly complementary sequence, and the sequence with a single mismatched base were designed according to the NOS1AP gene sequence. The detection probes were hybridized with their complementary oligonucleotides and single-base-mismatch oligonucleotides, respectively. Then, gold nanoparticles solutions and NaCl solutions were added, respectively. Different color appeared obviously by naked eye which showed an evident change in UV-Vis absorption spectroscopy similarly. The results indicate that with the presence of the appropriate concentration of NaCl, the gold nanoparticles solutions with double-stranded oligonucleotides are distinguished from gold nanoparticles solutions with single mismatched oligonucleotides by colormetric assay. This method enables a simple, rapid and efficient detection that could have potential and possible application prospects in medical diagnosis.

Key words: Gold nanoparticle, NOS1AP, Single nucleotide polymorphism, Colorimetric assay

TrendMD:

GUO Qing-Chuan, WANG Xiang, LOU Xin-Hui, MAO Hong-Ju*, JIA Jie*, JIN Qing-Hui, ZHAO Jian-Long, ZHANG Feng. Label-free Colorimetric Assay Based on Gold Nanoparticles in Detection of NOS1AP with Single Nucleotide Polymorphism[J]. Chem. J. Chinese Universities, 2010, 31(10): 1965.

References

[1]Syvanen A. C.. Nature Reviews Genetics[J], 2001, 2(12): 930—942 [2]Suzuki M. Y. O., Sekiya T., Hayashi K.. Genomics[J], 1989, 5: 874—879 [3]Buetow K. H., Edmonson M., MacDonald R., et al.. Proc. Natl. Acad. Sci.[J], 2001, 98(2): 581—584 [4]Hsu T. M., Law S. M., Duan S. H., Neri B. P., Kwok P. Y.. Clinical Chemistry[J], 2001, 47(8): 1373—1377 [5]CAI Hong(蔡宏), WANG Yan-Qin(王延琴), HE Pin-Gang(何品刚), FANG Yu-Zhi(方禹之). Chem. J. Chinese Universities(高等学校化学学报)[J], 2003, 24(8): 1390—1394 [6]Li H., Rothberg L.. Proc. Nat. Acad. Sci.[J], 2004, 101: 14036—14039 [7]Cho K. N., Lee Y. H., Lee C. H., Lee K. T., King Y. M., Choi H. W., Ryu P. D., Lee S. Y., Joo S. W.. Phys. Chem. C[J], 2008, 112(23): 8629—8633 [8]FENG Rong-Fang(冯荣芳), HU Yu-Yan(胡玉燕), LI Wen-Bin(李文斌), LIU Hui-Qing(刘惠卿), LI Qing-Jun(李清君), ZHANG Min(张敏). Chin. J. Appl. Physiol.(中国应用生理学杂志)[J], 2009, 25(2): 182—185 [9]Aarnoudse A. J., Newton-Cheh C., de Bakker P. I., Straus S., Kors J. A., Hofman A., Uitterlinden A. G., Witteman J., Stricker B.. Circulation[J], 2007, 116: 10—16 [10]Lehtinen A. B., Newton-Cheh C., Ziegler J. T., Langefeld C. D., Freedman B. I., Daniel K. R., Herrington D. M., Bowden D. W.. Diabetes[J], 2008, 57: 1108—1114 [11]Eijgelsheim, Mark. Aarnoudse, Adrianus L. H. J. Rivadeneria, et al.. Human Molecular Genetics[J], 2009, 18(2): 347—357 [12]Grabar K. C., Freeman R. G., Hommer M. B., Natan M. J.. Anal. Chem.[J], 1995, 67(4): 735—743 [13]Mirkin C. A., Letsinger R. L., Mucic R. C., Storhoff J. J.. Nature[J], 1996, 382: 607—609 [14]Joo S. W.. Chem. Lett.[J], 2004, 33: 60—61 [15]Weisbecker C. S., Merritt M. V., Whitesides G. M.. Langmuir[J], 1996, 12: 3763—3772 [16]Wang L., Liu X., Hu X., Song S., Fan C.. Chem. Commun.[J], 2006, 36: 3780—3782

Label-free Colorimetric Assay Based on Gold Nanoparticles in Detection of NOS1AP with Single Nucleotide Polymorphism

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	BAI Cuiting, YUE Renye, LUO Liegao, MA Nan. Quantitative Analysis of MicroRNA Content by Fluorescence Imaging in Cancer Cells Using Dual-color Fluorescence Nanosensor ^† [J]. Chem. J. Chinese Universities, 2020, 41(6): 1252.
[2]	WANG Tingting, LI Yuan, YANG Lili, BAO Changhao, CHENG Han. In vivo Dynamic Detection of Aloe Polysaccharides Using Carbon Fiber Microelectrodes Modified with Gold Nanoparticles ^† [J]. Chem. J. Chinese Universities, 2020, 41(1): 87.
[3]	ZHU Qinfu,HU Kezhen,LI Xiaojie,CHEN Mingqing. Preparation of Dendrimer-gold Nanoparticle Composites for pH Responsive Drug Delivery^† [J]. Chem. J. Chinese Universities, 2019, 40(5): 1065.
[4]	LI Ying, KANG Junjun, ZHAO Xueru, XU Wenkai, QI Qi. Preparation of Gold-modified Magnetic Graphene-based Molecularly Imprinted Composites and Electrochemical Sensing Detection of Dinbutyl Phthalate in Water^† [J]. Chem. J. Chinese Universities, 2019, 40(3): 448.
[5]	Siqi SUN,Ying WANG,Chuanyin SUN,Runwei WANG,Zhendong ZHANG,Zongtao ZHANG,Shilun QIU. Preparation and Catalytic Performance of Bowl-shaped Amphiphilic ZSM-5 Zeolites Supported Gold Nanoparticles ^† [J]. Chem. J. Chinese Universities, 2019, 40(12): 2436.
[6]	HE Caimei,ZHENG Jingyi,LI Xiaoxia. MoS₂-Gold Nanoparticles and Thionine-gold Nanoparticle Based Signal-enhanced Electrochemical Aptasensor for the Detection of 17β-Estradiol ^† [J]. Chem. J. Chinese Universities, 2019, 40(10): 2090.
[7]	FENG Wei,WANG Bowei,ZHENG Yan,JIANG Yang. Preparation and Surface-enhanced Raman Scattering(SERS) of Single Au Nanodot^† [J]. Chem. J. Chinese Universities, 2018, 39(9): 1875.
[8]	LI Aiju, WANG Yuxi, LU Shaoyong, LIU Kun. Ligand Exchange of Gold Nanoparticles with Thiol-terminated Polystyrene^† [J]. Chem. J. Chinese Universities, 2018, 39(3): 552.
[9]	WANG Li, LI Zhi, SHEN Xiaoqin, MA Nan. Programming Single Quantum Dot Valencies via DNA Caging^† [J]. Chem. J. Chinese Universities, 2018, 39(1): 32.
[10]	ZHANG Chanchan, ZHANG Fanghui, DING Lei, NI Zhenjie, JIANG Lang, DONG Huanli, ZHANG Xiaotao, LI Rongjin, HU Wenping. Organic Phototransistor Based on Surface Plasmon Resonance Effect^† [J]. Chem. J. Chinese Universities, 2018, 39(1): 102.
[11]	HUANG Haiping, YUE Yafeng, XU Liang, LÜ Lianlian, HU Yongmei. Glucose Biosensor Based on Dy₂(MoO₄)₃-AuNPs Composite Nanomaterial^† [J]. Chem. J. Chinese Universities, 2017, 38(4): 554.
[12]	MENG Qingnan, WANG Kai, TANG Yufei, ZHAO Kang, XIANG Siyuan, ZHANG Kai, ZHAO Lang. Preparation of Gold Nanoparticles Loaded Hollow Silica Spheres and Their Catalytic Performance^† [J]. Chem. J. Chinese Universities, 2017, 38(3): 503.
[13]	LI Dan, WU Qian, LIU Li, SUN Xiaori, GUO Meng, FENG Yimin. Preparation and Stability of Gemini Surfactant Modified Gold Nanofluids^† [J]. Chem. J. Chinese Universities, 2017, 38(10): 1829.
[14]	ZHAO Tiantian, CHEN Yuqing, ZHANG Min, WANG Yuerong, ZHANG Hongyang, HU Ping. Fabrication of Paper-based Microfluidic Chips and the Application on the Determination of Uric Acid in Serum Based on Gold Nanoparticle-assisted Catalysis^† [J]. Chem. J. Chinese Universities, 2016, 37(5): 829.
[15]	HUANG Lijun, ZHANG Lihua, MAO Hongju, WANG Ping, JIANG Youxu, LI Panpan, JIN Qinghui, ZHAO Jianlong. Detection of Myocardial Injury Markers Based on Double Gold Nanoparticles Probes and Protein Chip^† [J]. Chem. J. Chinese Universities, 2015, 36(9): 1687.