Immobilization of DNA Hydrogel Plugs in Glass Microfluidic Channels and Its Applications

Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (6): 1128.

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Immobilization of DNA Hydrogel Plugs in Glass Microfluidic Channels and Its Applications

ZHANG Yu^1,2, YU Hao¹, DONG Xiu-Ling³, QIN Jian-Hua¹*, LIN Bin-Cheng¹*

1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
2. Graduate School of Chinese Academy of Sciences, Beijing 100049, China;
3. Dalian Third Municipal Hospital, Dalian 116033, China

Received:2009-02-16 Online:2009-06-10 Published:2009-06-10
Contact: QIN Jian-Hua, E-mail: bclin@dicp.ac.cn; LIN Bin-Cheng, E-mail: jhqin@dicp.ac.cn
Supported by:
国家自然科学基金(批准号: 20635030, 20575067)和科技部“八六三”计划(批准号: 2006AA02Z305)资助.

Abstract

Abstract:

A method of DNA probes immobilization and specific target sequences capture in a microfluidic chip was presented. Acrydite-modified DNA probes are immobilized in silanized glass microfluidic channels via photopolymerization in a polyacrylamide matrix. The resulting polymeric, hydrogel plugs are porous under electrophoretic conditions, and the immobilized DNA probes can be hybridized with fluorescence labeled complementary DNA. The total analysis process can be completed within 5 min, and the limit of detection is 0.1 μmol/L. This method is simple, rapid and feasible. The double-stranded DNA can be chemically denatured, and the chip is reusable. The conditions for photopolymerization, hybridization, and denaturation were discussed as well.

Key words: Microfluidic chip, Acrylamide hydrogel, DNA probes immobilization, Hybridization

TrendMD:

ZHANG Yu, YU Hao, DONG Xiu-Ling, QIN Jian-Hua*, LIN Bin-Cheng*. Immobilization of DNA Hydrogel Plugs in Glass Microfluidic Channels and Its Applications[J]. Chem. J. Chinese Universities, 2009, 30(6): 1128.

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Immobilization of DNA Hydrogel Plugs in Glass Microfluidic Channels and Its Applications

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