Designment of a New Self-assembling Peptide D-EAK16 with D Amino Acid into 3D Nanofiber Scaffold

Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (6): 1131.

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Designment of a New Self-assembling Peptide D-EAK16 with D Amino Acid into 3D Nanofiber Scaffold

YU Zu-Xiao¹, CHEN Jia², LUO Zhong-Li³*

1. Department of Material and Chemical Engineering, Sichuan University of Science & Engineering, Zigong 643000, China;
2. Biomaterial Lab, Engineering Research Centre for Waste Oil Recovery Technology and Equipment, Ministry of Education, Chongqing Technology and Business University, Chongqing 400067, China;
3. West China Hospital, Laboratory for Nanobiomedical Technology, Sichuan University, Chengdu 610065, China

Received:2008-09-11 Online:2009-06-10 Published:2009-06-10
Contact: LUO Zhong-Li, E-mail: zhongliluo@163.com
Supported by:
国家“九八五”-Ⅱ期平台项目和四川省材料腐蚀与防护重点实验室项目(批准号: 2008CL06)资助.

Abstract

Abstract:

A new generation self-assembling peptide as a research model was synthesized with D amino acid. Circular dichroism, AFM and cell 3D culture were used to study the objective model, D-EAK16. The result reveal that a stable secondary structure of D-EAK16 is β-sheet at 30 ℃. D-EAK16 can form transparent hydrogel with 99% water content, which can self-assemble to nanofibers and to the scaffold in physiological solution such as PBS and DMEM. Toxicity of the ECM(extracellular matrices) was not found when cultured the cell stains HO-8910 and SPC-A-1 in the solution of D-EAK16. Moreover, there is no significant difference to toxicity between the nano-scaffolds made of D or L amino acid. We hope these synthetic peptides can be as a 3D microenvironment of the cells(just similar to the bodies), widely use in the biomedical engineering.

Key words: D Amino acid, D-Peptide, Self-assembling peptide, Nanofiber, Cell-scaffold

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YU Zu-Xiao, CHEN Jia, LUO Zhong-Li*. Designment of a New Self-assembling Peptide D-EAK16 with D Amino Acid into 3D Nanofiber Scaffold[J]. Chem. J. Chinese Universities, 2009, 30(6): 1131.

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Designment of a New Self-assembling Peptide D-EAK16 with D Amino Acid into 3D Nanofiber Scaffold

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