Rapid Detection of Trace Organophosphorus Pesticides by Membrane Enrichment Combined with Membrane Image Colorimetric†

doi:10.7503/cjcu20180799

Abstract

Abstract:

A new method was developed for the rapid detection of organophosphorus pesticides based on smartphone and multi-channel membrane enrichment. According to the property that the activity of acetylcholinesterase was reduced by the specific inhibition of organophosphorus pesticides, indole acetate was used as a substrate which can be hydrolyzed by enzyme to form indigo. Indigo was enriched on a nylon membrane by the multi-channel membrane enrichment device with which analyte in standard solutions and sample solutions can be enriched on the same membrane at different positions. A mobile APP was developed to detect the membrane. Digital photographs of membrane were collected by the phone. With the APP, the influence of illumination and noise was eliminated and all samples enriched at different positions on the membrane could be identified automatically, the color signal of the samples were extracted, thus a standard curve of the color signal vs. concentration of the pesticide could be regressed. Limit of detection of trichlorfon was estimated being 0.030 mg/L. The content of trichlorfon in cucumber was analyzed with the developed method. The results showed that the recoveries were 95.8%—106.9% with relative standard deviations of 3.8%—5.4%. Using a smartphone but spectrometer, the proposed method is simple and applicable. Comparing with the method of GB/T 5009.100-2003, sensitivity and accuracy are improved significantly. The method showed potential applications in fast detection fields.

Key words: Smartphone, Membrane enrichment, Image colorimetric, Trichlorphon

CLC Number:

O652

TrendMD:

XIONG Qin,YANG Wuye,YANG Shaojie,LIU Jing,YANG Shihua,CHEN Wanchao,LI Long,DU Yiping. Rapid Detection of Trace Organophosphorus Pesticides by Membrane Enrichment Combined with Membrane Image Colorimetric^†[J]. Chem. J. Chinese Universities, 2019, 40(6): 1135.

Figures/Tables 7

Scheme 1 Detection principle based on the inhibition of acetylcholinesterase

Fig.1 Digital photograph of a membrane with enriched analyst

Fig.2 Relationship between diffuse reflectance spectrum and concentration of trichlorphon

Fig.3 Effect of pH value on absorbance ability

Fig.4 Effect of inhibition time on inhibition ratio of AChE

Fig.5 Relationship between inhibition rate of AChE and concentration of trichlorphon by Vis DRS method(A) and partial enlargement of (A)(B)

Table 1 Results of trichlorphon detection in cucumber by Vis DRS and DIC methods

Method	Added/(mg·L^-1)	Found/(mg·L^-1)	Recovery(%)	RSD(%, n=5)
Vis DRS	0.20	0.187	93.7	4.3
	0.50	0.544	108.9	4.2
	0.70	0.676	96.5	3.4
DIC	0.20	0.203	101.5	5.2
	0.50	0.534	106.9	3.8
	0.70	0.670	95.8	5.4

References 18

[1]	Stachniuk A., Szmagara A., Czeczko R., Fornal E., Journal of Environmental Science and Health,2017, 52(7), 446-457
[2]	Hong Z. C., Xiong H. H., Liu Y. Y., Jian D. W., Liang X. X., Food Research & Development,2018, 39(10), 129-132
[3]	Bernardi G., Kemmerich M., Ribeiro L. C., Adaime M. B., Zanella R., Prestes O. D., Talanta,2016, 161, 40-47
[4]	Standardization Administration of the People’s Republic of China, Rapid Determination for Organophosphate and Carbamate Pesticide Residues in Vegetables, GB/T 5009.100-2003
	(中国人民共和国国家标准化管理委员会. 蔬菜中有机磷和氨基甲酸酯类农药残留量的快速检测, GB/T 5009.100-2003)
[5]	Zhang Y., Li L., Su L. Q., Chu H. T., Sun L., Qin X., Du Y. P., Chinese Journal of Analytical Chemistry,2018, 46(5),729—734(张圆, 李龙, 苏立强, 初洪涛, 孙琳, 覃潇, 杜一平. 分析化学, 2018, 46#(5), 729-734)
[6]	Wei H. Y., Li H., Liu P., Tao X., Fan C. C., Li L., Zhang R. Q., Du Y. P., Spectroscopy Letters,2017, 50(9), 470-475
[7]	Wang L., Cao P., Li W., Tong P. J., Zhang X. F., Du Y. P., Spectrochimica Acta Part A Molecular & Biomolecular Spectroscopy,2016, 159, 151-156
[8]	Li W., Zhang R. Q., Wang H. T., Jiang W., Wang L., Li H., Wu T., Du Y. P., Analytical Methods,2014, 8(14), 2887-2894
[9]	Li W., Wang L., Tong P. J., Iqbal J., Zhang X. F., Wang X., Du Y. P., RSC Adv., 2014, 4(94), 52123-52129
[10]	Zhang X. F., Zhu B. L., Li W., Wang L., Zhang L., Wu T., Du Y. P., Spectroscopy & Spectral Analysis,2015, 35(7), 1944-1948
[11]	Chen G. P., Hu H. L., Wu T., Tong P. J., Liu B. X., Zhu B. L., Du Y. P., Food Control,2014, 35(1), 218-222
[12]	Yin C.H., Iqbal J., Hu H. L., Liu B. X., Zhang L., Zhu B. L., Du Y. P.,Journal of Hazardous Materials, 2012, 233-234
[13]	Iqbal J., Yin C. H., Geng J. P., Li X. Y., Li X. Y., Du Y. P., Microchimica Acta,2012, 177(1), 195-200
[14]	Guo J., Wong J. X., Cui C., Li X., Yu H. Z., Analyst,2015, 140(16), 5518-5525
[15]	Parastar H., Shaye H., Chemometrics & Intelligent Laboratory Systems,2015, 147, 105-110
[16]	Peng B., Chen G., Li K., Zhou M., Zhang J., Zhao S., Food Chem.,2017, 230, 667-672
[17]	Wang L., Li W., Zhang F. Y., Li H., Cai G. M., Li B., Qian G. B., Du Y. P., Journal of Biological & Chemical Luminescence,2016, 31(6), 1187-1193
[18]	Li Y.Q., Modern Enzymatic Analysis, Beijing Medical University& Peking Union Medical College United Press, Beijing, 1994, 32-33
	(李毓琦. 现代酶法分析北京: 北京医科大学中国协和医科大学联合出版社, 1994, 32-33)