Effect of Phase Behavior of Phospholipids on Lipid Membrane Damage Induced by Graphene Oxide †

doi:10.7503/cjcu20190645

Abstract

Abstract:

The difference among different kinds of phospholipids in biomembrane not only lies in different structures in polar head groups, but also in different alkyl chain structures which lead to different phase states. Phase state of phospholipids plays an important role in cell activities. In this study, phospholipid membranes with different phase states were constructed by using two phospholipids with different phase transition temperatures and adjusting their mixing ratio, to reveal the effect of the phase behavior of the phospholipid membrane on the interaction between graphene oxide and the phospholipid membrane. Results showed that, for the extraction of phospholipid from membrane, graphene oxide exhibited significant phase selectivity, and it selectively extracted phospholipids in fluid phase. More importantly, the extraction effect of graphene oxide on fluid-phase phospholipids was affected by the presence of gel-phase phospholipids in the membrane. The extraction could occur only when the fluid-phase phospholipid molecules accounted for the majority of the phospholipids in the membrane, and only the phospholipids in fluid phase were extracted. Therefore, the extraction effect of graphene oxide on phospholipids in membrane not only had fluid-phase selectivity, but also was regulated by the phase behavior of phospholipid membrane.

Key words: Phospholipid membrane, Phase behavior, Graphene oxide, Lipid extraction effect

CLC Number:

O652

TrendMD:

ZHANG Xiaofei, WU Lie, LI Shanshan, ZHU Manyu, CHENG Xiaowei, JIANG Xiu’e. Effect of Phase Behavior of Phospholipids on Lipid Membrane Damage Induced by Graphene Oxide ^†[J]. Chem. J. Chinese Universities, 2020, 41(4): 661.

Figures/Tables 7

Fig.1 TEM image(A), AFM image(B, inset shows the height profile), UV-Vis spectrum(C), survey(D), C1s(E) XPS spectra and FTIR spectrum(F) of GO sample

Fig.2 SEIRAS of GO interacting with lipid membrane in single phase state(A) DOPC lipid membrane in fluid state; (B), (C) DPPC lipid membrane in gel state and in fluid state in water, respectively. Sample spectra were obtained with the spectra of lipid membrane in water as reference. Curves a—f were recorded at 1, 5, 10, 30, 60 and 90 min, respectively.

Fig.3 Fluorescence images of the morphology change of GO interacting with lipid membrane in single phase state(A), (A') DOPC lipid membrane in fluid state before(A) and after(A') interacting with GO. (B), (B'), (C), (C') DPPC lipid membrane in gel state(B, B') and in fluid state(C, C') before(B, C) and after(B', C') interacting with GO.

Fig.4 Cartoon schematic diagram of orientation of head group of PC lipids in gel phase and fluid phase(A) and fluorescence intensity change of fluorescence-labeled DOPC and DPPC vesicles after interacting with GO(B)

Fig.5 SEIRA difference spectra of GO interact with DPPC/DOPC(1∶10)(A), DPPC/DOPC(1∶4)(B) and DPPC/DOPC(1∶1)(C) lipid membranes in two phase separation Sample spectra were obtained with the spectra of lipid membrane in water as reference, respectively. ^Curves a—f were recorded at 1, 5, 10, 30, 60 and 90 min, respectively.

Fig.6 Fluorescence images of the morphology change of DPPC/DOPC(1∶10)(A, D), DPPC/DOPC(1∶4)(B, E) and DPPC/DOPC(1∶1)(C, F) lipid membrans interact without(A—C) and with(D—F) GO in two phase separation

Fig.7 Normalized emission spectra(A) and generalized polarization GP340 value(B) of lipid vesicles mixed with different molar ratios of DOPC and DPPC lipids

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