Fluorescence Detection Based on DNA-templated Silver Nanoclusters and the Construction of Multi-level Logic Gate †

doi:10.7503/cjcu20190385

Abstract

Abstract:

Fluorescent silver nanoclusters were synthesized using DNA as template(DNA-Ag NCs), and characterized by fluorescence, ultraviolet and infrared spectroscopy. According to the fluorescence changes generated by the interaction between DNA-Ag NCs and ions, relative concentration detections can be carried out. Under the optimal experimental conditions, the concentrations of Ni ²⁺ and Hg ²⁺ showed a linear relationship with the fluorescence intensity of DNA-Ag NCs. The practicability of this fluorescent probe in tap water samples is also verified. Since DNA-Ag NCs can respond to a variety of stimuli, including Ni ²⁺, S ^2-, Hg ²⁺ and H ⁺(pH=7.0), logic gates of multiple input and their combination can be constructed based on the corresponding fluorescence intensity. When definition of fluorescence output intensity(I_output)>initial fluorescence intensity(I_origin) as output=1 was adopted, the YES, INH and integrative NOR and INH logic gates were constructed. And if I_output≥I_origin is defined as output=1, the NOT, NOR and integrative IMP, NOR and AND logic gates can be established. Based on DNA-Ag NCs, complex logic gates involving multiple inputs can be constructed to realize the transformation and transmission of chemical information, which has great application prospects in constructing new molecular devices.

Key words: Fluorescence detection, DNA template, Silver nanoclusters, Logic gate

CLC Number:

O652

TrendMD:

XIAO Zhourui,HUANG Donghua,BIAN Mengmeng,YUAN Yali,NIE Jinfang. Fluorescence Detection Based on DNA-templated Silver Nanoclusters and the Construction of Multi-level Logic Gate ^†[J]. Chem. J. Chinese Universities, 2020, 41(1): 102.

Figures/Tables 12

Fig.1 UV-Vis(a), fluorescence excitation(b) and emission(c) spectra of DNA-Ag NCs(A), infrared spectra of DNA(a) and DNA-Ag NCs(b)(B), TEM images of DNA-Ag NCs with different magnifications(C, D)

Fig.2 Fluorescence(A) and UV-Vis absorption spectra(B) of DNA-Ag NCs(a) with Ni2+(b) and Hg2+(c) Inset of (A): fluorescence intensity change of DNA-Ag NCs with the addition of Hg2+ and Ni2+, respectively.

Fig.3 Fluorescence intensities of DNA-Ag NCs with Hg2+(a) and Ni2+(b) at different time interval

Fig.4 Fluorescence spectra of DNA-Ag NCs with Hg2+(A) and Ni2+(B) Insets: linear relationship between fluorescence intensity and concentration of ions.

Ion	Spiked/(μmol·L^-1)	Detected/(μmol·L^-1)	Recovery(%)	RSD(%)
Hg²⁺	0.02	0.018	90.0	4.7
	0.08	0.078	97.5	1.2
	0.14	0.160	114.2	4.3
Ni²⁺	0.04	0.044	110.0	1.1
	0.15	0.164	109.3	1.9
	0.29	0.299	103.1	2.0

Fig.5 Influence of different factors on fluorescence of DNA-Ag NCs a. Cd2+; b. Cr3+; c. Cu2+; d. Hg2+; e. Ni2+; f. pH=3.0; g. pH=7.0; h. S2-; i. Cl-; j. Pb2+; k. Al3+.

Fig.6 Ni2+ as unitary input YES logic gate (A) Fluorescence spectra of DNA-Ag NCs with different inputs; (B) fluorescence intensity of YES gate; (C) logic gates symbol; (D) truth table.

Fig.7 Ni2+ and Hg2+ as binary input INH logic gate (A) Fluorescence spectra of DNA-Ag NCs with different input; (B) fluorescence intensity of INH gate; (C) logic gates symbol; (D) truth table.

Fig.8 Ni2+, Hg2+ and S2- as ternary input integrative NOR and INH logic gate (A) Fluorescence spectra of DNA-Ag NCs with different inputs; (B) fluorescence intensity; (C) logic gates symbol; (D) truth table.

Fig.9 S2- as unitary input NOT logic gate (A) Fluorescence spectra of DNA-Ag NCs with different inputs; (B) fluorescence intensity of NOT gate; (C) logic gates symbol; (D) truth table.

Fig.10 Hg2+ and S2- as binary input NOR logic gate (A) Fluorescence spectra of DNA-Ag NCs with different inputs; (B) fluorescence intensity of NOR gate; (C) logic gates symbol; (D) truth table.

Fig.11 Ni2+, H+, Hg2+ and S2- as quaternary input integrative IMP, NOR and AND logic gate (A) Fluorescence spectra of DNA-Ag NCs with different input combinations; (B) fluorescence intensity; (C) logic gates symbol; (D) truth table.

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