Chemical Journal of Chinese Universities ›› 2020, Vol. 41 ›› Issue (11): 2324-2334.doi: 10.7503/cjcu20200405

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Structural Modification of Fluorescent Proteins and Their Applications in Biosensing

LI Huiyuan, LEI Chunyang, HUANG Yan(), NIE Zhou()   

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology,Hunan University,Changsha 410082,China
  • Received:2020-06-30 Online:2020-11-10 Published:2020-11-06
  • Contact: HUANG Yan,NIE Zhou;
  • Supported by:
    the National Natural Science Foundation of China(21675044)


Since the discovery of fluorescent proteins, they have played important roles in life science, due to their genetic coding and spontaneous emission of robust fluorescence. With the development of protein engineering technology and organic synthesis, and a better understanding of the structure and function of fluorescent proteins, structural modifications and designs of fluorescent proteins were performed to give them new properties and functions, and broaden their applications in biological field such as bioimaging and biosensing. This paper focuses on the progress of the structural modification of green fluorescent protein(GFP), as well as its mimics, including mutagenesis, barrel structure reconstruction, surface reconstruction, and nucleic acid mimics of fluorescent proteins. The representative applications of these fluorescent proteins and mimics in biosensing are also introduced.

Key words: Fluorescent protein, Structural modification, Surface reconstruction, Nucleic acid mimic of fluorescent protein, Biosensing

CLC Number: