Chem. J. Chinese Universities ›› 2021, Vol. 42 ›› Issue (11): 3445.doi: 10.7503/cjcu20210450

• Review • Previous Articles     Next Articles

Research Progress on RNA⁃cleaving DNAzyme for the Detection of Pathogenic Bacteria

MAO Yu(), QU Hao, ZHENG Lei()   

  1. School of Food and Biological Engineering,Hefei University of Technology,Hefei 230009,China
  • Received:2021-06-30 Online:2021-11-10 Published:2021-11-10
  • Contact: ZHENG Lei E-mail:maoyu@hfut.edu.cn;lei.zheng@aliyun.com
  • Supported by:
    the National Natural Science Foundation of China(32072306)

Abstract:

DNAzymes, produced through in vitro selection process, are single-stranded oligonucleotides that have catalytic functions. With the development of in vitro selection technology, more and more DNAzymes have been selected. Among them, RNA-cleaving DNAzyme(RCD) has the functions of molecular recognition and catalytic signal amplification, and has the advantages of flexible design, easy preparation and low modification cost, etc., which has gained widespread attention and application in the field of detection of pathogenic bacteria, providing a new molecular tool for early detection and prevention of pathogenic bacteria. In this paper, the research and application of RNA-cleavage deoxyribozyme in the detection of pathogenic bacteria were systematically summarized, and the problems and future development prospects of RNA-cleavage deoxyribozyme were also prospected.

Key words: DNAzyme, RNA-cleaving, Detection, Pathogenic bacteria

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