Chem. J. Chinese Universities ›› 2021, Vol. 42 ›› Issue (8): 2393.doi: 10.7503/cjcu20210280

• Analytical Chemistry • Previous Articles     Next Articles

Preparation of Paper Hollow Gold Nanocage SERS Sensor and Its Rapid and Highly Sensitive Detection for miRNAs in Sputum of Patients with Non-small Cell Lung Cancer

XUE Jin1, CAO Xiaowei2(), LIU Yifan3(), WANG Min1   

  1. 1.Guangling College,Yangzhou University,Yangzhou 225001,China
    2.Institute of Translational Medicine,Medical College,Yangzhou University,Yangzhou 225001,China
    3.The First Clinical College,Dalian Medical University,Dalian 116000,China
  • Received:2021-04-25 Online:2021-08-10 Published:2021-08-05
  • Contact: LIU Yifan E-mail:cxw19861121@163.com;liuyifan0667@sina.com
  • Supported by:
    the National Natural Science Foundation of China(81701825);the Natural Science Program of Guang?ling College of Yangzhou University, China(ZKYB180016)

Abstract:

The abnormal expression of nucleic acid is closely related to the occurrence and development of tumor. Detecting the expression of micro-RNA(miRNAs) markers is of great significance for the diagnosis and treatment of non-small cell lung cancer(NSCLC). In this work, the monolayer hollow gold nanocages(HGNCs) array substrate was fabricated by the self-assembly method at the oil-water interface. It was proved that the HGNCs gap provided a large number of “hot spots” by FDTD method, which made the substrate exhibit an excellent surface-enhanced Raman scattering(SERS) performance. At the same time, the hairpin DNA labeled by Raman signal molecule was linked with the substrate, and then the SERS signal was detected after complementary hybridization with the target miRNAs. The detection limits of miR-196a and miR-21 in sputum were 10.00 and 36.15 amol/L, respectively. In order to verify the accuracy of the sensor in clinical detection, the SERS sensor was used to detect miR-196a and miR-21 in sputum of patients with NSCLC, and the results were compared with those of real-time quantitative polynucleotide chain reaction technology. There were no significant differences between the results of the two detection methods, which both showed that the expressions of miR-196a and miR-21 in sputum of NSCLC patients were higher than those of healthy people, and the relative standard deviations were less than 10%. Therefore, the paper HGNCs SERS sensor has potential application in NSCLC diagnosis, and may become an alternative tool for miRNAs research in biomedical diagnosis field.

Key words: Hollow gold nanocages, Self-assembly at oil-water interface, Surface-enhanced Raman spectroscopy(SERS), Flexible substrate

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