Microfluidic Analysis System Integrated with Liquid-liquid Extraction and Liquid-liquid Waveguide†

doi:10.7503/cjcu20150701

Abstract

Abstract:

A microfluidic analysis system integrated with liquid-liquid extraction and liquid-liquid waveguide detection was explored. Multiphase laminar liquid-liquid extraction was realized using 1-butyl-3-methyl imidazolium bromide ionic liquid/sodium carbonate solution aqueous two-phase system. Liquid-liquid waveguide absorption photometric detection was achieved by the ionic liquid with higher refractive index served as liquid core and the salt aqueous solution with lower refractive index served as liquid cladding. The extraction efficiency for cresol red extraction is more than 93%. The linear range, the relative standard deviation and the detection limit for cresol red detection were 0.01—0.40 mg/mL, 3.4%(n=11) and 3.8 μg/mL(3σ) respectively. Extraction separation and liquid-liquid waveguide-based long optical path absorbance detection were integrated in the system, which provided a new strategy for expanding the application of absorbance detection in microfluidic systems.

Key words: Microfluidic chip, Ionic liquid aqueous two-phase extraction, Liquid-liquid waveguide, Absorbance detection, Integration

CLC Number:

O657.32

TrendMD:

JIANG Jian, LI Panpan, MA Yingxue, YANG Chunguang, XU Zhangrun. Microfluidic Analysis System Integrated with Liquid-liquid Extraction and Liquid-liquid Waveguide^†[J]. Chem. J. Chinese Universities, 2016, 37(4): 648.

Figures/Tables 5

Fig.1 Channel layout of the microfluidic chip(A), multiphase flows at different positions of the extraction channel(B—E) and photograph of the microfluidic chip(F) (A) 1. Inlet of the liquid core; 2 and 3. inlets of the inner cladding liquid; 4 and 5. inlets of the outer cladding liquid.

Fig.2 Schematic diagram of liquid-liquid waveguide in the detection channela. Liquid core; b. liquid cladding; c. optical fiber for transmitting incident light; d. optical fiber for receiving optical signal.θc: critical angle of the total internal reflection.

Fig.3 Effect of incident angle of the optical fiber on absorbance

Fig.4 Standard curve of cresol red in IL solution

Table 1 Extraction of cresol red in the Na2CO3 solution

No.	c₁/(mg·mL^-1)	c₂/(mg·mL^-1)	c₂'/(mg·mL^-1)	Extraction rate(%)	RSD(%)(n=10)
1	0.0103	0.0241	0.0258	93.4	8.9
2	0.0516	0.128	0.129	99.2	3.5
3	0.103	0.259	0.258	100	1.8

References 17

[1]	Collins G. E., Lu Q., Pereira N., Wu P., Talanta, 2007, 72(1), 301—304
[2]	Liang Z. H., Chiem N., Ocvirk G., Tang T., Fluri K., Harrison D. J., Anal. Chem., 1996, 68(6), 1040—1046
[3]	Petersen N. J., Mogensen K. B., Kutter J. P., Electrophoresis, 2002, 23(20), 3528—3536
[4]	Mishra S. K., Dasgupta P. K., Anal. Chim. Acta, 2007, 605(2), 166—174
[5]	Salimi-Moosavi H., Jiang Y. T., Lester L., McKinnon G., Harrison D. J., Electrophoresis, 2000, 21(7), 1291—1299
[6]	Okada T., Electrophoresis, 2007, 28(19), 3414—3419
[7]	Du W. B., Fang Q., Fang Z. L., Chem. J. Chinese Universities, 2004, 25(4), 610—613
	(杜文斌, 方群, 方肇伦. 高等学校化学学报, 2004, 25(4), 610—613)
[8]	Sun M., Du W. B., Fang Q., Talanta, 2006, 70(2), 392—396
[9]	Wolfe D. B., Conroy R. S., Garstecki P., Mayers B. T., Fischbach M. A., Paul K. E., Prentiss M., Whitesides G. M., Pro. Natl. Acad. Sci. USA, 2004, 101(34), 12434—12438
[10]	Lee K. S., Kim S. B., Lee K. H., Sung H. J., Kim S. S., Appl. Phys. Lett., 2010, 97(2), 021109
[11]	Lim J. M., Urbanski J. P., Choi J. H., Thorsen T., Yang S. M., Anal. Chem., 2011, 83(2), 585—590
[12]	Zhao D. H., Zeng Y. B., Li L., Zou J. J., Zheng X. J., Shen B., Xi Q. H., Chinese J. Anal. Chem., 2009, 37(3), 445—448
	(赵弟海, 曾延波, 李蕾, 邹建军, 郑先军, 沈兵, 奚奇辉. 化学进展, 2009, 37(3), 445—448)
[13]	Claudio A. F. M., Freire M. G., Freire C. S. R., Silvestre A. J. D., Coutinho J. A. P., Sep. Purif. Technol., 2010, 75(1), 39—47
[14]	Chen X.W., Mao Q. X., Wang J. H.,Prog. Chem., 2013, 25(5), 661—668
	(陈旭伟, 毛全兴, 王建华. 化学进展, 2013, 25(5), 661—668)
[15]	Zeng Q., Wang Y. Z., Li N. Huang X., Ding X. Q., Lin X., Huang S. Y., Liu X. J., Talanta, 2013, 116, 409—416
[16]	Lin X., Wang Y. Z., Zeng Q., Ding X. Q., Chen J., Analyst, 2013, 138(21), 6445—6453
[17]	Novak U., Pohar A., Plazl I., Znidarsic-Plazl P., Sep. Purif. Technol., 2012, 97, 172—178