Incubation and Activity Determination of Lactate Dehydrogenase Isoenzymes on Microfluidic Chip

Chem. J. Chinese Universities

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Incubation and Activity Determination of Lactate Dehydrogenase Isoenzymes
on Microfluidic Chip

LIU Jing^1,2; ZHUANG Gui-Sheng^1,2; JIA Chun-Ping¹; JIN Qing-Hui¹;
WANG Hui-Min³; ZHAO Jian-Long^1,4; YANG Meng-Su^1*

1. Shanghai Institute of Microsystem and Information Technology, Chinese
Academy of Sciences, Shanghai 200050, China;
2. Graduate School of Chinese Academy of Sciences, Beijing 100039, China;
3. Affiliated Hospital of Nantong University, Nantong 226001, China;
4. National Engineering Research Center for Shanghai Biochip Technology,
Shanghai 201203, China

Received:2005-08-22 Revised:1900-01-01 Online:2006-07-10 Published:2006-07-10
Contact: YANG Meng-Su

Abstract

Abstract: A novel incubation and activity determination method of lactate dehydrogenase isoenzymes was established. Based on the on\|chip electrophoretic separation theory the analyzer was integrated with a temperature\|controlling system. The assay program of the enzymes including injection of samples, incubation and separation was highly performed in the microchannel. A system for high sensitivity fluorescence detection was developed. A 360 nm light source was adopted to excite the fluorescence and the 460 nm signature was finally selectively gathered. The efficient separation of different LDH fractions indicates the potential of microfluidic devices for isoenzymes assay and the detection limit reached 0.5 U/L.

Key words: Microfluidic chip, Temperature-controlling, Lactate dehydrogenase isoenzymes, Activity determination

CLC Number:

O652.6

TrendMD:

LIU Jing^1,2; ZHUANG Gui-Sheng^1,2; JIA Chun-Ping¹; JIN Qing-Hui¹;
WANG Hui-Min³; ZHAO Jian-Long^1,4; YANG Meng-Su^1*. Incubation and Activity Determination of Lactate Dehydrogenase Isoenzymes
on Microfluidic Chip[J]. Chem. J. Chinese Universities, doi: .

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Incubation and Activity Determination of Lactate Dehydrogenase Isoenzymes
on Microfluidic Chip

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