Anti-complementary Activities of a (1→6) Linked Glucan from Korean Mondshood Root and Its Sulfated Derivatives†

doi:10.7503/cjcu20131268

Abstract

Abstract:

In order to study anti-complement activity in the classical pathway of Korean Mondshood root and its sulfated polysaccharides, a homogeneous polysaccharide(KMPS-2A) was gained from the Korean Mondshood root[Aconitum coreanum(Lévl.) Raipaics] by water extract and alcohol precipitate via DE-32, Superdex-200 and Superdex-75 gel filtration. Its structure was investigated by high performance gel permeation chromatography, methylation analysis, NMR and IR spectrometry. The results showed that the average molecular weights(M_w) of the KMPS-2A was 676000 and it was consisted of a repeating structure as [α-1,6-D-Glc]_n. Sulfated derivatives were gained by chlorosulfonic acid-pyridine method and it was found that the sulfated derivatives had the highest degree of substitution(1.79) when the volume ratio of chlorosulfonic acid and pyridine was 1.75∶1.0. It was shown that sulfated polysaccharide 1.75B had a better anti-complementary activity. C2, C3 and C4 were substituted by sulfate groups. A certain correlation was presented between the degree of substitution and the anticomplementary activity of sulfated derivatives. Anticomplementary activity of sulfated derivative 1.75B was better than the heparin group in the classical pathway, which suggested the potential activity of the complement inhibitors.

Key words: Polysaccharide, Korean Mondshood root, Complement

CLC Number:

O629.12

TrendMD:

LI Xiaojun, JIANG Jiaye, SHI Songshan, LI Yuan, JIANG Yongbo, KE Yan, WANG Shunchun. Anti-complementary Activities of a (1→6) Linked Glucan from Korean Mondshood Root and Its Sulfated Derivatives^†[J]. Chem. J. Chinese Universities, 2014, 35(7): 1423.

Figures/Tables 3

References 25

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	(Ed.: J, H, D, K)

Compound	δ
Compound	C1	C2	C3	C4	C5	C6	C1'	C2s	C3s	C4s	C5'
Native(KMPS-2A)	99.4	73.2	74.9	71.27	71.9	66.78
Sulfated(1.75B)	98.24		76.88	71.27	70.4	67.15	97.0	77.58	80.2	75.9	69.26

Compound	δ
Compound	C1	C2	C3	C4	C5	C6	C1'	C2s	C3s	C4s	C5'
Native(KMPS-2A)	99.4	73.2	74.9	71.27	71.9	66.78
Sulfated(1.75B)	98.24		76.88	71.27	70.4	67.15	97.0	77.58	80.2	75.9	69.26

Sample	DS^a	10^-4	C/(μg·mL^-1)
KMPS-2A		67.6	1250±1.84
Heparin			7.81±1.10
0.25B	0.57	75.2	812±2.19
0.5B	0.85	23.3	496±1.59
0.75B	0.92	11.1	610±1.41
1.0B	1.12	5.75	784±3.95
1.5B	1.24	4.27	790±2.22
1.75B	1.79	2.38	3.77±1.30

Sample	DS^a	10^-4	C/(μg·mL^-1)
KMPS-2A		67.6	1250±1.84
Heparin			7.81±1.10
0.25B	0.57	75.2	812±2.19
0.5B	0.85	23.3	496±1.59
0.75B	0.92	11.1	610±1.41
1.0B	1.12	5.75	784±3.95
1.5B	1.24	4.27	790±2.22
1.75B	1.79	2.38	3.77±1.30