Structural Characterization of One Homogeneous Polysaccharide from Echinops latifolius Tausch. and Anti-complementary Activity of Its Sulfated Derivatives†

doi:10.7503/cjcu20140089

Abstract

Abstract:

To isolate and characterize the anti-complementary polysaccharide from the root of Echinops latifolius Tausch., bioactivity-guided fractionation and purification was used to obtain the anti-complementary polysaccharide from the hot-water extract of the root of Echinops latifolius Tausch. The polysaccharide was characterized by various chemical and spectral analyses. The anti-complementary activities were evaluated by hemolytic assay in vitro. The action targets were identified in the system with individual complement-depleted sera. A homogenous water-soluble polysaccharide(EPS-2A) was obtained from Echinops latifolius Tausch., which average molecular weight was estimated to be 118000. A combination of monosaccharide composition, methylation and configuration analysis, as well as NMR spectroscopy, indicated that EPS-2A was poly-(1-4)-α-D-galactopyranosyluronic acid in which (87.8±0.5)% of uronic acid existed as methylester. Two sulfated derivatives(Sul-2A-1 and Sul-2A-2) from EPS-2A were prepared after sulfation with 1∶1 and 2∶1 of chlorosulfonic acid and pyridine, respectively. The anti-complementary assay showed that Sul-2A-1 and Sul-2A-2 demonstrated a stronger inhibitory effect [CH₅₀=(74.1±4.6) μg/mL for Sul-2A-1; CH₅₀=(35.7±2.8) μg/mL for Sul-2A-2] on the complement activation through the classic pathway, compared to that of heparin [CH₅₀=(103.0±9.0) μg/mL]. The results suggested that the sulfated derivatives Sul-2A-1 and Sul-2A-2 might be promising drug candidates in case of necessary therapeutic complement inhibition.

Key words: Echinops latifolius Tausch., Polysaccharide, Structure analysis, Sulfated derivative, Anti-complement

CLC Number:

O629.12

TrendMD:

BAO Bin, WANG Huijun, WANG Hongwei, SHI Songshan, WANG Shunchun. Structural Characterization of One Homogeneous Polysaccharide from Echinops latifolius Tausch. and Anti-complementary Activity of Its Sulfated Derivatives^†[J]. Chem. J. Chinese Universities, 2014, 35(10): 2119.

Figures/Tables 2

References 23

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