Solution, Conformational Properties and Morphology of Psyllium Polysaccharide with Ferulic Acid Removal†

doi:10.7503/cjcu20150513

Abstract

Abstract:

This study was aimed at investing the solution and conformation properties of polysaccharide from psyllium(or seeds of Plantago asiatica L.). The fraction which was subjected with NaOH to remove ferulic acid was named as PLCP-FAS, while another fraction was PLCP-FAE whose ferulic acid was removed by feruloyl esterase. Ubbelohde capillary viscometer, HPSEC with refractive index, light scattering and viscometric detectors and scanning electron microscope(SEM) were applied to investigate the two fractions’ solution, conformation properties and solid form. The results showed that there was little change in monosaccharide compositions of the polysaccharide after ferulic acid removing, not mater by NaOH or enzyme. Sugar contents and uronic acid contents were much higher both for the two fractions. However, its apparent viscosity reduced remarkably. Intrinsic viscosity of PLCP-FAS and PLCP-FAE were close either in 0.1 mol/L NaCl or 0.5 mol/L NaOH. HPSEC analysis results showed that weight average molecular weight of PLCP-FAS was 3.17×10⁶, while it was 2.83×10⁶ for PLCP-FAE. Persistence length of PLCP-FAS and PLCP-FAE were similar. SEM results showed that both PLCP-FAE and PLCP-FAS still exhibited certain linear chain characteristics. The above results indicated that the psylliun polysaccharide after removing ferulic acid was semi flexible, and morphology of the polysaccharide remained some linear chain characteristics.

Key words: Psyllium, Polysaccharide, Ferulic acid, Solution property, Conformation characteristic

CLC Number:

O629.12

TrendMD:

YIN Junyi, LIU Xiaoying, NIE Shaoping, XIE Mingyong. Solution, Conformational Properties and Morphology of Psyllium Polysaccharide with Ferulic Acid Removal^†[J]. Chem. J. Chinese Universities, 2016, 37(1): 43.

Figures/Tables 5

References 27

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Fraction	Sugar content(%)	Uronic acid content(%)	Monosaccharide composition(%)
Fraction	Sugar content(%)	Uronic acid content(%)	Rhamnose	Arabinose	Xylose	Glucose	Galactose
PLCP-FAS	82.49	20.34	2.34±0.09	21.62±0.66	71.77±0.14	ND	4.28±0.42
PLCP-FAE	80.83	19.41	1.85±0.29	22.73±0.85	72.19±0.62	ND	3.20±0.53

Fraction	Sugar content(%)	Uronic acid content(%)	Monosaccharide composition(%)
Fraction	Sugar content(%)	Uronic acid content(%)	Rhamnose	Arabinose	Xylose	Glucose	Galactose
PLCP-FAS	82.49	20.34	2.34±0.09	21.62±0.66	71.77±0.14	ND	4.28±0.42
PLCP-FAE	80.83	19.41	1.85±0.29	22.73±0.85	72.19±0.62	ND	3.20±0.53

Fraction	Mass recovery(%)	[η]/(dL·g^-1)	10^-6 M_w	10^-6 M_n	R_g/nm	R_h/nm
PLCP-FAS	68.7±6.6	3.03±0.30	3.17±0.38	0.96±0.19	113.3±7.6	48.2±3.5
PLCP-FAE	65.8±0.6	3.02±0.02	2.83±0.14	0.86±0.02	124.9±3.8	45.7±0.7

Fraction	Mass recovery(%)	[η]/(dL·g^-1)	10^-6 M_w	10^-6 M_n	R_g/nm	R_h/nm
PLCP-FAS	68.7±6.6	3.03±0.30	3.17±0.38	0.96±0.19	113.3±7.6	48.2±3.5
PLCP-FAE	65.8±0.6	3.02±0.02	2.83±0.14	0.86±0.02	124.9±3.8	45.7±0.7