Isolation, Structural Characterization and IEC-6 Cell Migration Activities of Polysaccharides From Atractylodes Macrocephala Koidz†

doi:10.7503/cjcu20140515

Abstract

Abstract:

Bioassay-guided fractionation led to isolation of a new polysaccharide YY13008 from the roots of Atractylodes macrocephala Koidz by aqueous extraction, ethanol precipitation and purification followed by column chromatography on DEAE-cellulose and sephadex LH-20. The chemical structure of polysaccharide YY13008 was then elucidated on the basis of acidic hydrolysis, environmental scanning electron microscope and spectroscopic data, including IR, 1D and 2D NMR analysis. The polysaccharide YY13008 was determined as homogeneous, with a molecular weight of 6546, as determined by a gel permeation chromatography-multi-angle laser light scattering(GPC-MALLS) method. The polysaccharide YY13008 was found to be a fructan containing almost exclusively a(2→1) -linked β-D-fructofuranosyl repeating units with terminal α-glucopyranosyl unit. The in vitro wound healing assays revealed that the treatment of polysaccharide YY13008 with difluoromethylornithine(DFMO), a specific inhibitor of ornithine decarboxylase, was able to completely reverse DFMO-induced inhibition of IEC-6 migration, however, the treatment of YY13008 alone did not affect the basal rate of IEC-6 migration.

Key words: Atractylodes macrocephala Koidz, Polysaccharide, Fructan, Cell migration

CLC Number:

O631.6

TrendMD:

WANG Yiyu, LIU Jiawei, SIMA Zhenhua, SONG Houpan, LI Ruliu, CAI Jiazhong, CHEN Weiwen. Isolation, Structural Characterization and IEC-6 Cell Migration Activities of Polysaccharides From Atractylodes Macrocephala Koidz^†[J]. Chem. J. Chinese Universities, 2015, 36(2): 299.

Figures/Tables 3

References 20

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Species	No.	Chicory inulin^[19]	δ, ¹³C NMR	HSQC(¹³C ×¹H)	COSY(¹H ×¹H)	HMBC(¹³C→¹H)
β-D-fructofuranosyl	1	60.33	60.8	3.64(d, J=0.8 Hz)		3-H
(2→1)				3.85(d, J=10.8 Hz)
	2	102.65	103.2			3-H, 1-Ha, 1-Hb
	3	76.42	77.1	4.19(d, J=8.5 Hz)	4-H	4-H, 1-Hb,1-Ha
	4	73.32	74.3	4.03(t, J=8.2 Hz)	3-H, 5-H	3-H,5-H,6-H
	5	80.5	81.1	3.79(m, +)	4-H, 6-H	4-H,6-H
	6	61.66	62.2	3.69(m, +)	5-H	4-H,5-H
Terminal	1	91.89	92.5	5.37(d, J=3.0 Hz)
α-D-glucopyranosyl	2	70.60	71.2	ND
(1→2)	3	72.03	72.6	ND
	4	68.66	69.2	3.40(t, J=9.6 Hz)
	5	71.85	72.4	3.48(m)
	6	59.57	61.0	ND
(Terminal α-D-glc→)	1		60.5	ND
β-D-fructofuranosyl	2		103.7	ND
(1→2)	3		77.3	ND
	4		73.9	ND
	5		81.3	ND
	6		62.3	ND

Species	No.	Chicory inulin^[19]	δ, ¹³C NMR	HSQC(¹³C ×¹H)	COSY(¹H ×¹H)	HMBC(¹³C→¹H)
β-D-fructofuranosyl	1	60.33	60.8	3.64(d, J=0.8 Hz)		3-H
(2→1)				3.85(d, J=10.8 Hz)
	2	102.65	103.2			3-H, 1-Ha, 1-Hb
	3	76.42	77.1	4.19(d, J=8.5 Hz)	4-H	4-H, 1-Hb,1-Ha
	4	73.32	74.3	4.03(t, J=8.2 Hz)	3-H, 5-H	3-H,5-H,6-H
	5	80.5	81.1	3.79(m, +)	4-H, 6-H	4-H,6-H
	6	61.66	62.2	3.69(m, +)	5-H	4-H,5-H
Terminal	1	91.89	92.5	5.37(d, J=3.0 Hz)
α-D-glucopyranosyl	2	70.60	71.2	ND
(1→2)	3	72.03	72.6	ND
	4	68.66	69.2	3.40(t, J=9.6 Hz)
	5	71.85	72.4	3.48(m)
	6	59.57	61.0	ND
(Terminal α-D-glc→)	1		60.5	ND
β-D-fructofuranosyl	2		103.7	ND
(1→2)	3		77.3	ND
	4		73.9	ND
	5		81.3	ND
	6		62.3	ND