Extraction and Identification of the Low Molecular Weight Proteins in Serum

Abstract

Abstract: An improved disc sodium dodecyl sulphate-polyacrylamide gel electrophoresis(SDS-PAGE) to extract low molecular weight(LMW) proteins from human and cutoff proteins larger than 3×104 was developed. In the disc SDS-PAGE, short column resolving gel and little of electrode buffer solutions were used in mini home-made electrophoresis equipment. Passive elution of the LMW proteins from the gel was adopted without staining. After removing SDS and precipitation, the obtained LMW protein mixtures were denatured directly in solution and degraded in the presence of trypsin to obtain peptides. The peptides were analyzed by HPLC/MS/MS. The LMW proteins were identified by matching the peptide patterns in mass spectra with those in Mascot data pool and 97 kinds of LMW proteins in human serum were identified.

Key words: Serum, Low molecular weight protein, HPLC/MS/MS, Disc PAGE

TrendMD:

LI Tie-Chun, WANG Zi-Ming, WANG Lu, ZHANG Han-Qi, YU Yong*. Extraction and Identification of the Low Molecular Weight Proteins in Serum[J]. Chem. J. Chinese Universities, 2010, 31(10): 1955.

References

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