Thermodynamic Study of Chiral Recognition Between Serum Albumin and Tryptophan Enantiomers by Surface Plasmon Resonance†

doi:10.7503/cjcu20141037

Abstract

Abstract:

The chiral recognition between serum albumin and L-/D-tryptophan was studied by surface plasmon resonance from the point of view of thermodynamic. The effects of pH, ionic strength of buffer solution and temperature on binding affinity were investigated in detail. The thermodynamic parameters were further evaluated and used to explore the chiral recognition mechanism. It has been found that both BSA and HSA had excellent chiral recognition capability to L- and D-tryptophan. Moreover, there were highly specific interactions between BSA(HSA) and L-tryptophan in comparison with D-tryptophan. The calculated results of thermodynamic parameters indicated that the hydrophobic interaction played a dominant role. In addition, electrostatic interaction may also certainly contribute to the interactions between L-tryptophan and serum albumin.

Key words: Surface plasmon resonance, Chiral recognition, Serum albumin, Tryptophan, Thermodynamic

CLC Number:

O657

TrendMD:

SHEN Gangyi, GAO Yan, ZHANG Aiqin, CUI Jian, DAI Dongsheng. Thermodynamic Study of Chiral Recognition Between Serum Albumin and Tryptophan Enantiomers by Surface Plasmon Resonance^†[J]. Chem. J. Chinese Universities, 2015, 36(5): 881.

Figures/Tables 4

Fig.1 Variation of association equilibrium constant(KA) of L-Trp(a) and D-Trp(b) with BSA as a function of pH at 25 ℃

Fig.2 Effect of NaCl concentration on the association equilibrium constant(KA) of L-Trp(a) and D-Trp(b) with BSA at 25 ℃ with pH=7.4

Fig.3 Effect of temperature on the association equilibrium constant(KA) of L-Trp(a) and D-Trp(b) with BSA at pH=7.4

Table 1 Thermodynamic parameters of interactions between L-Trp and D-Trp with BSA and HSA(pH=7.4) under 15—35 ℃

Protein	Analyte	ΔH/(kJ·mol^-1)	ΔS/(kJ·mol^-1·K^-1)	R²	ΔΔH/(kJ·mol^-1)	ΔΔS/(kJ·mol^-1·K^-1)
BSA	L-Trp	53.80	0.2252	0.9949	9.38	0.0397
	D-Trp	44.42	0.1855	0.9421
HSA	L-Trp	44.10	0.1925	0.9428	2.25	0.0194
	D-Trp	41.85	0.1732	0.9950

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