Enzyme Immobilization Technique in Microfluidic Channel on Integrated Chip

Abstract

Abstract: A new method for the immobilization of enzyme in microfluidic channel on the integrated chip for proteomic analysis was reported. The PDMS film immersing in the acrylic acid solution was irradiated under UV lamp to produce active free radicals and then immersed alternatively into the PDDA solution and trypsin solution for self-assemble. The monomer solution of the produced chip was characterized with the reflectance IR technique, contact angle determination and SEM observations. A standard protein BSA was selected to test the performance of the product. It was verified that CE and MALDI-TOF/TOF-MS detection can be on-line carried out in a single, successive running.

Key words: Microfluidic chip, Immobilized enzyme, PDMS

CLC Number:

O657

TrendMD:

TIAN Yu-Ping, WU Hui-Ling, CHEN Shu-Gui, YANG Peng-Yuan . Enzyme Immobilization Technique in Microfluidic Channel on Integrated Chip[J]. Chem. J. Chinese Universities, 2004, 25(5): 847.

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Enzyme Immobilization Technique in Microfluidic Channel on Integrated Chip

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