Chem. J. Chinese Universities ›› 2014, Vol. 35 ›› Issue (1): 1.doi: 10.7503/cjcu20130691
• Review • Previous Articles Next Articles
ZHU Lina, ZHU Ying*(), FANG Qun*
Received:
2013-07-22
Online:
2014-01-10
Published:
2013-12-11
Contact:
ZHU Ying,FANG Qun
E-mail:yingzhu@zju.edu.cn
Supported by:
CLC Number:
TrendMD:
ZHU Lina, ZHU Ying, FANG Qun. Recent Progress of Microfluidic Techniques for Protein Crystallization and Screening†[J]. Chem. J. Chinese Universities, 2014, 35(1): 1.
Fig.1 PDMS-based microvalve for protein crystallization under FID mode(A) Schematic illustration of PDMS microvalves under open(A1) and close(A2) states; (B) CCD image of microchip designed to implement three FID assays at three mixing ratios; (C) optical micrographs of crystals grown in the microchamber of FID chip[24].
Fig.2 PDMS peristaltic micropump and formulator chip(A)Schematic diagram of an PDMS peristaltic micropump[22]; (B) CCD image of the formulator chip[27].
Fig.3 Droplet-based microfluidic system for protein crystallization(A) Schematic diagram showing the procedures of droplet formation in a T junction channel[36]; (B) Protein crystallization with vapor diffusion method in a PDMS/glass-capillary composite microchip[35]; (C) a high throughput screening system using an on-chip integrated sampling probe and a slotted-vial array; (C1)—(C3) sample introduction process; (C4) image of a generated droplet array containing different samples in the chip[20].
Fig.4 DropLab for protein crystallization[21](A) Principle and procedure of DropLab for droplet generation; (B) setup of the DropLab system with slotted-vial array for sample presentation and microcapillaries for droplet storage; (C) protein crystallization screening result using DropLab.
Fig.5 SlipChip-based protein crystallization[46](A) Operation procedures of SlipChip; (B) crystals of the photosynthetic reaction center from Blastochloris viridis obtained with SlipChip.
Fig.6 Microfluidic system for membrane protein crystallization within lipidic mesophases(LCP)(A1) CCD image showing the mixing of bacteriorhodopsin solution(left and right chambers) with lipid monoolein(center chamber) in a microfluidic chip[63]; (A2) crystals of bacteriorhodopsin obtained in LCP; (B) membrane protein crystals grown in droplets; (B1) bacteriorhodopsin from Halobacterium salinarum; (B2) caro-tenoid-containing RC from Rhodobacter sphaeroides; (B3) caroteniodless RC from Rhodobacter sphaeroides; (B4) photosynthetic reaction center from Rhodopseudomonas viridis[65].
[1] | Mcpherson A., Methods, 2004, 34(3), 254—265 |
[2] | Chayen N. E., Curr. Opin. Struct., 2004, 14(5), 577—583 |
[3] | Chayen N. E., Trends Biotechnol., 2002, 20(3), 98 |
[4] | Berman H. M., Bhat T. N., Bourne P. E., Feng Z., Gilliland G., Weissig H., Westbrook J., Nat. Struct. Mol. Biol., 2000, 7, 957—959 |
[5] | Chayen N. E., J. Cryst. Growth, 1999, 198, 649—655 |
[6] | Kuhn P., Wilson K., Patch M. G., Stevens R. C., Curr. Opin. Chem. Biol., 2002, 6(5), 704—710 |
[7] | Stevens R. C., Curr. Opin. Struct. Biol., 2000, 10(5), 558—563 |
[8] | Chayen N.E., Protein Crystallization Strategies for Structural Genomics, International University Line, California, 2007, 36—40 |
[9] | Nakanishi K., Sakiyama T., Imamura K., J. Biosci. Bioeng., 2001, 91(3), 233—244 |
[10] | Thudi L., Jasti L. S., Swarnalatha Y., Fadnavis N. W., Mulani K., Deokar S., Ponrathnam S., Colloids Surf. B Biointerfaces, 2012, 90, 184—190 |
[11] | Juárez-Martínez G., Steinmann P., Roszak A. W., Isaacs N. W., Cooper J. M., Anal. Chem., 2002, 74(14), 3505—3510 |
[12] | Sanjoh A., Tsukihara T., J. Cryst. Growth, 1999, 196(2), 691—702 |
[13] | Li L., Ismagilov R. F., Annu. Rev. Biophys., 2010, 39, 139—158 |
[14] | Bian T. B., Yin X. F., Liu J. H., Chem. Res. Chinese Universities, 2010, 26(4), 522—526 |
[15] | Hansen C., Quake S. R., Curr. Opin. Struct. Biol., 2003, 13(5), 538—544 |
[16] | Sauter C., Dhouib K., Lorber B., Cryst. Growth Des., 2007, 7(11), 2247—2250 |
[17] | Li J. J., Chen Q., Li G., Zhao J. L., Zhu Z. Q., Prog. Chem., 2009, 21(5), 1034—1039 |
(李俊君, 陈强, 李刚, 赵建龙, 朱自强.化学进展, 2009,21(5), 1034—1039) | |
[18] | Chen D. L., Ismagilov R. F., Curr. Opin. Chem. Biol., 2006, 10(3), 226—231 |
[19] | Zheng B., Gerdts C. J., Ismagilov R. F., Curr. Opin. Struct. Biol., 2005, 15(5), 548—555 |
[20] | Sun M., Fang Q., Lab Chip, 2010, 10(21), 2864—2868 |
[21] | Du W. B., Sun M., Gu S. Q., Zhu Y., Fang Q., Anal. Chem., 2010, 82(23), 9941—9947 |
[22] | Unger M. A., Chou H. P., Thorsen T., Scherer A., Quake S. R., Science, 2000, 288(5463), 113—116 |
[23] | Mcpherson A., Crystallization of Biological Macromolecules, Cold Spring Harbor Laboratory Press, New York, 1999, 455—460 |
[24] | Hansen C. L., Skordalakes E., Berger J. M., Quake S. R., Proc. Natl. Acad. Sci. USA, 2002, 99(26), 16531—16536 |
[25] | Bird R. B., Stewart W. E., Lightfoot E. N., Transport Phenomena, Wiley, New York ,1960, 138, 148 |
[26] | Hansen C. L., Classen S., Berger J. M., Quake S. R., J. Am. Chem. Soc., 2006, 128(10), 3142—3143 |
[27] | Hansen C. L., Sommer M. O., Quake S. R., Proc. Natl. Acad. Sci. USA, 2004, 101(40), 14431—14436 |
[28] | Anderson M. J., Hansen C. L., Quake S. R., Proc. Natl. Acad. Sci. USA, 2006, 103(45), 16746—16751 |
[29] | Thorsen T., Roberts R. W., Arnold F. H., Quake S. R., Phys. Rev. Lett., 2001, 86(18), 4163—4166 |
[30] | Chen J. S., Jiang J. H., Chin. J. Anal. Chem., 2012, 40(8), 1293—1300 |
(陈九生, 蒋稼欢.分析化学, 2012,40(8), 1293—1300) | |
[31] | Velev O. D., Prevo B. G., Bhatt K. H., Nature, 2003, 426(6966), 515—516 |
[32] | Burns M. A., Johnson B. N., Brahmasandra S. N., Handique K., Webster J. R., Krishnan M., Sammarco T. S., Man P. M., Jones D., Heldsinger D., Mastrangelo C. H., Burke D. T., Science, 1998, 282(5388), 484—487 |
[33] | Song H., Tice J. D., Ismagilov R. F., Angew. Chem. Int. Ed., 2003, 42(7), 768—772 |
[34] | Roach L. S., Song H., Ismagilov R. F., Anal. Chem., 2005, 77(3), 785—796 |
[35] | Zheng B., Tice J. D., Roach L. S., Ismagilov R. F., Angew. Chem. Int. Ed., 2004, 43(19), 2508—2511 |
[36] | Zheng B., Roach L. S., Ismagilov R. F., J. Am. Chem. Soc., 2003, 125(37), 11170—11171 |
[37] | Zheng B., Tice J. D., Ismagilov R. F., Anal. Chem., 2004, 76(17), 4977—4982 |
[38] | Srinivasan V., Pamula V. K., Fair R. B., Anal. Chim. Acta, 2004, 507(1), 145—150 |
[39] | Shestopalov I., Tice J. D., Ismagilov R. F., Lab Chip, 2004, 4(4), 316—321 |
[40] | Dolega M. E., Jakiela S., Razew M., Rakszewska A., Cybulski O., Garsteckil P., Lab Chip, 2012, 12(20), 4022—4025 |
[41] | Yadav M. K., Gerdts C. J., Sanishvili R., Smith W. W., Roach L. S., Ismagilov R. F., Kuhn P., Stevens R. C., J. Appl. Cryst., 2005, 38(6), 900—905 |
[42] | Zheng B., Ismagilov R. F., Angew. Chem. Int. Ed., 2005, 44(17), 2520—2523 |
[43] | Garstecki P., Gitlin I., Diluzio W., Whitesides G. M., Kumacheva E., Stone H. A., Appl. Phys. Lett., 2004, 85(13), 2649—2651 |
[44] | Lau B. T. C., Baitz C. A., Dong X. P., Hansen C. L., J. Am. Chem. Soc., 2006, 129(3), 454—455 |
[45] | Shim J. U., Cristobal G., Link D. R., Thorsen T., Jia Y., Piattelli K., Fraden S., J. Am. Chem. Soc., 2007, 129(28), 8825—8835 |
[46] | Du W. B., Li L., Nichols K. P., Ismagilov R. F., Lab Chip, 2009, 9(16), 2286—2292 |
[47] | Li L., Du W. B., Ismagilov R. F., J. Am. Chem. Soc., 2010, 132(1), 106—111 |
[48] | Li L., Du W. B., Ismagilov R. F., J. Am. Chem. Soc., 2010, 132(1), 112—119 |
[49] | Dhouib K., Malek C. K., Pfleging W., Gauthier-Manuel B., Duffait R. , Thuillier G., Ferrigno R., Jacquamet L., Ohana J., Ferrer J. L., Theobald-Dietrich A., Giege R., Lorber B., Sauter C., Lab Chip, 2009, 9(10), 1412—1421 |
[50] | Pinker F., Brun M., Morin P., Deman A. L., Chateaux J. F., Olieéric V., Stirnimann C., Lorber B., Terrier N., Ferrigno R., Sauter C., Cryst. Growth Des., 2013, 13(8), 3333—3340 |
[51] | Ng J. D., Gavira J. A., García-Ruíz J. M., J. Struct. Biol., 2003, 142(1), 218—231 |
[52] | Li G., Chen Q., Li J., Hu X., Zhao J., Anal. Chem., 2010, 82(11), 4362—4369 |
[53] | Luo Y. H., Li G., Chen Q., Zhao J. L., Chem. J. Chinese Universities, 2012, 33(10), 2178—2183 |
(罗娅慧, 李刚, 陈强, 赵建龙.高等学校化学学报, 2012,33(10), 2178—2183) | |
[54] | Zhou X. X., Lau L. N., Lam W. W. L., Au S. W. N., Zheng B., Anal. Chem., 2007, 79(13), 4924—4930 |
[55] | Drews J., Science, 2000, 287(5460), 1960—1964 |
[56] | Sanders C. R., Myers J. K., Annu. Rev. Biophys. Biomol. Struct., 2004, 33, 25—51 |
[57] | Deisenhofer J., Epp O., Miki K., Huber R., Michel H., Nature, 1985, 318(6047), 618—624 |
[58] | Loll P. J., J. Struct. Biol., 2003, 142(1), 144—153 |
[59] | Chang G., Spencer R. H., Lee A. T., Barclay M. T., Rees D. C., Science, 1998, 282(5397), 2220—2226 |
[60] | Nollert P., Prog. Biophys. Mol. Biol., 2005, 88(3), 339—357 |
[61] | Li L., Mustafi D., Fu Q., Tereshko V., Chen D. L. L., Tice J. D., Ismagilov R. F., Proc. Natl. Acad. Sci. USA, 2006, 103(51), 19243—19248 |
[62] | Caffrey M., Cherezov V., Nat. Protoc., 2009, 4(5), 706—731 |
[63] | Perry S. L., Roberts G. W., Tice J. D., Gennis R. B., Kenis P. J., Cryst. Growth Des., 2009, 9(6), 2566—2569 |
[64] | Khvostichenko D. S., Kondrashkina E., Perry S. L., Pawate A. S., Brister K., Kenis P. J. A., Analyst, 2013, 138(18), 5384—5395 |
[65] | Li L., Fu Q., Kors C. A., Stewart L., Nollert P., Laible P. D., Ismagilov R. F., Microfluid. Nanofluid., 2010, 8(6), 789—798 |
[1] | LIU Shasha, ZHANG Heng, YUAN Shiling, LIU Chengbu. Molecular Dynamics Simulation of Pulsed Electric Field O/W Emulsion Demulsification [J]. Chem. J. Chinese Universities, 2021, 42(7): 2170. |
[2] | CUI Yingtao, WANG Shun, LI Wei, CUI Shumin, HUANG Yanjie, LI He, DUAN Hu, SONG Meirong, DONG Zhichao, WANG Yilin, JIANG Lei. Research Progress in Droplet Deposition on Superhydrophobic Plant Leaves [J]. Chem. J. Chinese Universities, 2021, 42(4): 1061. |
[3] | KUANG Xiaojun, YI Jingwei, FANG Xiaoxia, LAI Dongmei, XU Hong. Preparation of Water-soluble Coumarin Fluorescent Substrate and Its Application in Droplet Based Digital Detection [J]. Chem. J. Chinese Universities, 2021, 42(11): 3537. |
[4] | LIU Xuejiao, YANG Fan, LIU Shuang, ZHANG Chunjuan, LIU Qiaoling. Progress in Aptamer-targeted Membrane Protein Recognition and Functional Regulation [J]. Chem. J. Chinese Universities, 2021, 42(11): 3277. |
[5] | HUANG Huilong, HUANG Hanxiong. Low-temperature Impact Behavior of Droplet on Injection-compression Molded Nanostructured PP/POE Blend Surfaces [J]. Chem. J. Chinese Universities, 2021, 42(10): 3195. |
[6] | PENG Huo, GAO Zehang, LIAO Chengyue, WANG Xiaodong, ZHOU Hongbo, ZHAO Jianlong. Robust Droplet Digital PCR Chip for Absolute Quantitative Detection of Nucleic Acid [J]. Chem. J. Chinese Universities, 2020, 41(8): 1760. |
[7] | DAI Haoyu,DONG Zhichao,JIANG Lei. Research Advance of Electrically Controlled Droplet Motion † [J]. Chem. J. Chinese Universities, 2020, 41(1): 1. |
[8] | LIN Yucai,PEI Wenle,SUN Ruoxuan,GAO Chunlei,CHEN Jipeng,ZHENG Yongmei. Droplet Condensation on Surfaces with Special Wettability† [J]. Chem. J. Chinese Universities, 2019, 40(6): 1236. |
[9] | WANG Yao,WANG Yunpeng,CAI Meirong,LI Huanrong,YANG Zhongqiang. Preparation and Growth of Interfacial Ultrasmall Water Droplets Between Alkanes and Hydrophobic Solid† [J]. Chem. J. Chinese Universities, 2018, 39(9): 2003. |
[10] | XU Dan,DING Yadan,WANG Xue,CONG Tie,LIU Junping,HONG Xia,PAN Ying. Microdroplet Detection of Protein Based on Superhydrophobic Polystyrene Film† [J]. Chem. J. Chinese Universities, 2018, 39(9): 1913. |
[11] | MENG Xiufeng,ZHAI Zhiwei,GUO Aijun. Self-assembled Monolayers for Controllable Adhesion of DOPA† [J]. Chem. J. Chinese Universities, 2018, 39(10): 2245. |
[12] | LI Dan, ZHENG Yongmei. Self-propelled Droplet Movement on Micro/nano Anisotropic Structures Surface† [J]. Chem. J. Chinese Universities, 2018, 39(1): 109. |
[13] | YUAN Haojun, GAO Wanlei, JING Fengxiang, LIU Songsheng, ZHOU Hongbo, JIA Chunping, JIN Qinghui, ZHAO Jianlong. Novel Microfluidic Droplet Digital PCR Chip for High Sensitive Detection of Nucleic Acid† [J]. Chem. J. Chinese Universities, 2017, 38(7): 1140. |
[14] | CAO Tingting, DU Shouwen, XU Wang, XING Bin, ZHAO Fei, WANG Maopeng, ZHU Yilong, BAI Jieying, TIAN Yufei, LIU Liming, ZHAO Cuiqing, ZHOU Yifa, LI Chang, JIN Ningyi. Establishment and Functional Analysis of MDCK Cell Line Induced IFITM3 Expression Based on Tet-On 3G System† [J]. Chem. J. Chinese Universities, 2017, 38(5): 770. |
[15] | YANG Meiyue, WANG Wei, TIAN Zhiqing, LI Yingying, YUAN Zhi. Preparation of Glycyrrhetinic Acid-modified Sodium Alginate Microgel Spheres for 3D Cell Culture† [J]. Chem. J. Chinese Universities, 2017, 38(2): 326. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||