A Novel Amperometric Immunosensor Based on Aggregation of Chitosan and CNBr-activated Alginate for Determining Schistosoma Japonicum

Abstract

Abstract: Anovel schistosoma japonicum(Sj) imunnosensor based on aggregation of chitosan and CNBr-activated alginate is developed.The complex formed by CNBr-activated alginate and antibody is aggregated on the surface of the paraffin-graphite-chitosan electrode by electrostatic adsorption(coacervation).The concentration of SjAg can be detected by determining the redox current of o-aminophenol oxidized by H₂O₂in the presence of HRP.The electrode can be reused by simple treatment.Moreover, the imunnosensor shows some improved performances including high sensitivity, selectivity and less non-specific adsorption.The sensor exhibits a linear response to SjAg in the concentration range from 0.64 to 40 μg/mL.The sensor was applied to the determination of SjAg in rabbit serum with satisfactory results.

Key words: CNBr-activated alginate, o-Aminophenol, Coacervation, Chitosan, Amperometric immunosensor

CLC Number:

O657

TrendMD:

CHAO Xuan, HU Shun-Qin, SHEN Guo-Li, YU Ru-Qin . A Novel Amperometric Immunosensor Based on Aggregation of Chitosan and CNBr-activated Alginate for Determining Schistosoma Japonicum[J]. Chem. J. Chinese Universities, 2003, 24(8): 1377.

[1]	XIA Wu, REN Yingyi, LIU Jing, WANG Feng. Chitosan Encapsulated CdSe QDs Assemblies for Visible Light-induced CO₂ Reduction in an Aqueous Solution [J]. Chem. J. Chinese Universities, 2022, 43(7): 20220192.
[2]	WANG Mingxia, LIU Zhihui, ZHU Zhen, LI Lingfeng, WANG Bowei. Preparation and Properties of Nano Lithium Magnesium Silicate-chitosan-sodium Alginate Composite Scaffold Materials [J]. Chem. J. Chinese Universities, 2021, 42(10): 3240.
[3]	WANG Bowei, MA Rui, WU Fan, LIU Zhihui, LI Lingfeng, ZHANG Xiao, LIU Dingkun, YANG Nan, LI Meihui, YANG Defeng, SUN Qi. Preparation and Characterization of Graphene Oxide-sodium Alginate-chitosan Composite Scaffold [J]. Chem. J. Chinese Universities, 2020, 41(9): 2099.
[4]	CAO Meng, LIU Yang, ZHANG Shangxi, WANG Zhenxi, XU Sheng. Synthesis and Photocatalytic Hydrogen Evolution Properties of Chitosan Cobalt Complex ^† [J]. Chem. J. Chinese Universities, 2020, 41(4): 735.
[5]	WANG Ruanfeng,YAN Shifeng,HU Zhen,YIN Jingbo. Preparation and Properties of CS/nHA Porous Composite Scaffold Based on In-situ Precipitation Method^† [J]. Chem. J. Chinese Universities, 2019, 40(5): 1080.
[6]	YIN Maoli,WANG Yingfeng,REN Xuehong. Preparation of Modified Chitosan Antibacterial Nanospheres^† [J]. Chem. J. Chinese Universities, 2019, 40(1): 173.
[7]	DING Lingyun, JU Yuanlai, SUN Wei, CHEN Ji. Preparation of Functional Patterned Protein Arrays via the Combination of Inverse Emulsion and Breath Figure Method^† [J]. Chem. J. Chinese Universities, 2018, 39(6): 1311.
[8]	LIU Zhihui,QIU Tianyuan,DU Liuyi,YANG Junxing,LIU Kang,WANG Bowei. Preparation and Characterization of Chitosan Alginate Plastic Scaffolds^† [J]. Chem. J. Chinese Universities, 2018, 39(5): 1105.
[9]	LI Hui, XING Zhe, LI Rong, ZHANG Mingxing, WANG Minglei, TAN Yizhe, WU Guozhong. Preparation of Cu²⁺-Chitosan Quaternary Ammonium Salt-g-Poly(acrylicacid) with Efficient Antibacterial Activity [J]. Chem. J. Chinese Universities, 2017, 38(5): 902.
[10]	LI Hong, DU Libo, ZHANG Han, ZHAO Yuming, LIU Yang. New Tanshinone IIA Delivery System Using Chitosan Nanomicelles of Small Size^† [J]. Chem. J. Chinese Universities, 2016, 37(7): 1229.
[11]	LI Zhengzheng, XU Ziyang, GAO Liuyi, ZENG Wei, ZHAO Linlin. Preparation and Characterization of Thermo-sensitive N-Acetyl Glycol Chitosan Hydrogel for Sustained Drug Release^† [J]. Chem. J. Chinese Universities, 2016, 37(12): 2299.
[12]	XIONG Xingquan, JIANG Yunbing, XIAO Shangyun, SHI Lin, SONG Sida. Highly Efficient Synthesis of 1,2,3-Triazoles Catalyzed by Schiff Base Functionalized Chitosan-CuBr Catalyst^† [J]. Chem. J. Chinese Universities, 2016, 37(10): 1863.
[13]	LIU Shuping, LIU Yichun, WANG Wei. Chitosan-assisted WO₃ Ultrathin Film and Electrochromic Performance^† [J]. Chem. J. Chinese Universities, 2015, 36(6): 1202.
[14]	YANG Junxing, WANG Qi, WANG Yuanyuan, HAN Shu, YAO Jiawei, SHAO Siqi, WANG Jinglong, LIU Zhihui, GUO Yupeng. Preparation and in vitro Release of Multilayer Alginate Chitosan Microspheres Loading VEGF and Vancomycin^† [J]. Chem. J. Chinese Universities, 2015, 36(5): 1025.
[15]	ZHANG Huanhuan, CHEN Jiyun, ZHOU Sunying. Preparation of Open Tubular Capillary Electrochromatography Column with Nano-chitosan Coating and Its Application for Basic Proteins Analyzing^† [J]. Chem. J. Chinese Universities, 2015, 36(4): 631.

A Novel Amperometric Immunosensor Based on Aggregation of Chitosan and CNBr-activated Alginate for Determining Schistosoma Japonicum

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments