Preparation and Antibacterial Properties of Ag/Fe3O4/rGO Nanocomposite†

doi:10.7503/cjcu20160753

Abstract

Abstract:

Antibacterial sliver/ferroferric oxide/reduced graphene oxide(Ag/Fe₃O₄/rGO) nanocomposite with magnetic separation performance was prepared by in situ reduction method with FeCl₂·4H₂O and AgNO₃, using NaBH₄ as reducing agent and graphene oxide(GO) as the carrier. The nanocomposite was characterized by X-ray powder diffraction(XRPD), X-ray photoelectron spectroscopy(XPS) and transmission electron microscopy(TEM). The results showed that the Fe₃O₄ and Ag nanoparticles were uniformly distributed on the rGO sheets. Hysteresis loop test results indicated excellent magnetism of the nanocomposite, the saturation susceptibility(M_s) was 40.5 A·m²·kg^-1. Adsorption and settling of magnetic separation in the bacteria liquid could be completed within 10 min by adding Ag/Fe₃O₄/rGO in magnetic field. Selecting Escherichia coli(E. coli) and Staphylococcus aureus(S. aureus) as the experimental strains, the antibacterial properties of the composite was evaluated by the agar diffusion method, minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC). Ag/Fe₃O₄/rGO showed strong antibacterial activities against E.coli and S.aureu. The antibacterial circle diameter was 18 mm and 13 mm, the MIC was 50 mg/L and 80 mg/L, and the MBC was 30 mg/L and 50 mg/L respectively.

Key words: Graphene oxide, Nanocomposite, Antibacterial, Magnetic separation

CLC Number:

O613
TB321

TrendMD:

DU Wenxiu, YANG Juan, SANG Yuxiang, ZHANG Lili, ZHAO Nan, XU Kai, CHENG Xiaonong. Preparation and Antibacterial Properties of Ag/Fe₃O₄/rGO Nanocomposite^†[J]. Chem. J. Chinese Universities, 2017, 38(3): 346.

Figures/Tables 11

Scheme 1 Preparation process and adsorption sedimentation of Ag/Fe3O4/rGO nanocomposite

Fig.1 XRD patterns of Ag/Fe3O4/rGO nanocomposite(A) and thermogravimetric curves of Fe3O4/rGO nanocomposite(B) The inset shows the XRD pattern of Fe3O4/rGO.

Fig.2 C1s(A), O1s(B), Ag3d(C) and Fe2p(D) XPS spectra of Ag/Fe3O4/rGO nanocomposite

Fig.3 TEM images of Fe3O4/rGO(A), Ag/Fe3O4/rGO(B), HRTEM image of Ag/Fe3O4/rGO(C) and STEM image of Ag/Fe3O4/rGO nanocomposite(D)

Fig.4 Magnetic hysteresis loop of Ag/Fe3O4/rGO nanocomposite The inset shows the magnetic separation of Ag/Fe3O4/rGO nanocomposite.

Fig.5 Adsorption sedimentation images of E. coli and S. aureus treated by Ag/Fe3O4/rGO nanocomposite for 1 min(A), 5 min(B) and 10 min(C), different bacterial liquid without magnetic field(D) and in magnetic field(E) for 1 h E: E. coli; S: S. aureus; N: Ag/Fe3O4/rGO nanocomposite; r: rGO.

Fig.6 Antibacterial activity of Fe3O4/rGO(500 mg/L), Ag(5 mg/L) and Ag/Fe3O4/rGO(500 mg/L) nanocomposite against E. coli in agar-well diffusion assay

Fig.7 Antibacterial activity of Ag/Fe3O4/rGO nanocomposite against E. coli(A) and S. aureus(B) in agar-well diffusion assay

Fig.8 Bacterial growth curves of E. coli(A) and S. aureu(B) streated by Ag/Fe3O4/rGO nanocomposite

Fig.9 Photographs of E. coli(A—C) and S. aureus(D—F) colonies on an agar plate treated by Ag/Fe3O4/rGO nanocomposite for 1 h(A, D), 2 h(B, E) and 3 h(C, F) (A)—(C) E. coli+30 mg/L Ag/Fe3O4/rGO; (D)—(F) S. aureus+50 mg/L Ag/Fe3O4/rGO.

Fig.10 Cell viability of human HepG2 cell line in the presence of different materials

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Preparation and Antibacterial Properties of Ag/Fe₃O₄/rGO Nanocomposite^†

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