Effect of Substrate Surface Wettability on the Adsorption of Magnetic Carrier/Protein Nanocomposites†

doi:10.7503/cjcu20170241

Abstract

Abstract:

Hydrophobic ZnO seed layer and superhydrophobic ZnO nanowire array were grown on glass substrate, respectively. The adsorption of the magnetic carrier/bovine serum albumin(BSA) nanocomposites could reach a maximum saturation level upon applying an external magnetic field. The magnetic separation efficiencies using the ZnO nanowire array as the separation substrate were proved to be much higher than those using glass or the ZnO seed layer as the separation substrate. And the difference became large with the decrease of the nanocomposite concentrations. It might be attributed to the small solid-liquid contact area of the superhydrophobic substrate and its strong flow shear. The target protein was then further expanded to the other two typical proteins with different properties, i.e., hemoglobin and lysozyme. And the results proved that the effect rule of the substrate surface wettability on protein adsorption was universal. The superhydrophobic substrate has a more obvious advantage in improving the magnetic separation efficiency than commercial glass, polypropy-lene and hydrophobic substrate, and exhibited outstanding magnetic separation efficiencies for “hard” proteins. The present work could contribute to a better understanding of the effect of the interfacial property on protein adsorption behavior, and open up a new avenue to fabricate highly efficient protein separation platform.

Key words: Protein, Adsorption, Wettability, Magnetic separation efficiency, Nano-ZnO

CLC Number:

O647

TrendMD:

HU Qian, DING Yadan, PAN Ying, HONG Xia. Effect of Substrate Surface Wettability on the Adsorption of Magnetic Carrier/Protein Nanocomposites^†[J]. Chem. J. Chinese Universities, 2018, 39(1): 124.

Figures/Tables 9

Fig.1 SEM images of glass slide(A), ZnO seed layer(B) and ZnO nanowire array(C)Insets are the photographs of water droplets on the corresponding surfaces.

Fig.2 Zeta potentials of magnetic carriers(A) and BSA(B), the particle sizes of magnetic carriers(C) and magnetic carrier/BSA nanocomposites(D)

Table 1 Zeta potentials and particle sizes of the magnetic carrier, BSA and their nanocomposites

Sample	Zeta potential/mV	Size/nm
Magnetic carrier	41.32±3.86	68.06±10.34
BSA	-16.28±12.78
Magnetic carrier/BSA		190.14±52.79

Fig.3 Schematic illustration of the magnetic separation process

Fig.4 Photographs of substrates during the separation process of the magnetic carrier/BSA nanocomposites under an external magnetic field(A) Before enrichment; (B) After enrichment;(C) After enrichment. (A1)—(C1) Glass;(A2)—(C2) ZnO seed layer; (A3)—(C3) ZnO nanowire array.

Fig.5 Enrichment efficiencies of the magnetic carrier/BSA nanocomposites upon applying an external magnetic field

Fig.6 Magnetic separation efficiencies of the magnetic carrier/BSA nanocomposites with varying concentrations using glass, ZnO seed layer and ZnO nanowire array as substrate

Fig.7 Magnetic separation efficiencies of the magnetic carrier/LZM nanocomposites(a) and the magnetic carrier/HGB nanocomposites(b) with varying concentrations using glass(A), ZnO seed layer(B) and ZnO nanowire array as substrates(C)

Fig.8 Magnetic separation efficiencies of the magnetic carrier/LZM nanocomposites, the magnetic carrier/BSA nanocomposites and the magnetic carrier/HGB nanocomposites with concentrations of 50 μg/mL using ZnO nanowire array and PP as substrateThe insets are the SEM image of PP and the photograph of water droplet on PP surface.

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