Polyethyleneimine Functionalized Graphene Oxide Against hIAPP Amyloid Aggregation†

doi:10.7503/cjcu20180166

Abstract

Abstract:

Preventing human islet amyloid polypeptide(hIAPP) fibrillogenesis has been a primary strategy in development of therapeutic drugs for type Ⅱ diabetes. In this study, we prepared stable GO-PEI complex by modification of polyetherimide(PEI) on the surface of graphene oxide(GO) using covalent binding method. The results of transmission electron microscopy(TEM) and circular dichroism(CD) show that GO-PEI is more efficient than GO in inhibiting hIAPP fibril formation. The ThT fluorescence assay and atomic force microscopy(AFM) image data show that GO-PEI is the most effective for the inhibition of hIAPP fibrillation when it is added at the initial stage of hIAPP aggregation, and less effective when added in the growing period of hIAPP aggregation, but the inhibitor disables to disrupt the matured hIAPP fibrils. The findings may provide information valuable to the research and development of GO-based inhibitors.

Key words: Graphene oxide, Human islet amyloid polypeptide, Inhibitor

CLC Number:

O631
O641.3

TrendMD:

LU Tong, WANG Chunyang, ZHU Zhihui, JIANG Wei, HUO Mingnan, LI Fei. Polyethyleneimine Functionalized Graphene Oxide Against hIAPP Amyloid Aggregation^†[J]. Chem. J. Chinese Universities, 2018, 39(6): 1274.

Figures/Tables 8

Fig.1 IR(A) and TGA(B) spectra of GO(a), GO-PEI(b) and PEI(c)

Fig.2 AFM images of GO(A) and GO-PEI(B)

Fig.3 Kinetics of hIAPP aggregation as monitored by the thioflavin T fluorescence(A) and far-UV CD spectra of hIAPP in the absence(a) and presence of GO(b) and GO-PEI(c)(B)

Fig.4 TEM images of hIAPP(A), hIAPP with GO(B) and GO-PEI(C)

Fig.5 Kinetics of hIAPP aggregation as monitored by the thioflavin T fluorescence(A) in the absence(a) and presence of PEI(b) and TEM image(B) of hIAPP in the presence of PEI

Table 1 Hydrodynamic diameter and Zeta potential of GO and GO-PEI

Sample	Diameter(DLS)/nm	Zeta potential/mV
GO	200—600	-10.5
GO-PEI	150—300	50.1

Fig.6 Aggregation process of hIAPP modulated by GO-PEI at various time

Fig.7 AFM images of hIAPP(A) and mixed with GO-PEI at the incubation time of 0 h(B), 5 h(C) and 8 h(D)All images were recorded after the mixtures were further incubated for 10 h.

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