Preparation and Biological Evaluation of Electrospun Nano-silver/Anisodamine/PLGA†

doi:10.7503/cjcu20150475

Abstract

Abstract:

Nano-silver powders(n-Ag)/Anisodamine(AD)/poly(lactide-co-glycolide)(PLGA) were prepared by electrospinning. Field emission scanning electron microscope(ESEM) and Fourier transform infrared spectroscopy(FTIR) were applied for the investigation of the microscopic structure of the dressings as well as drug release, antibacterial and biological functions so that we could comprehensively evaluate the functionality of the dressings. The results show that the fibers exhibit a fully interconnected pore structure with narrow pore size distribution. And the diameters of electrospun fibers are influenced by the incorporation of AD. The result of drug release test proves that anisodamine can gradually release. It is also found that the n-Ag/AD/PLGA dressings exhibit a well antibacterial ability. The cell experimental results show that when the content of AD is from 1% to 5%, the dressings are non-toxic and contribute to the growth and proliferation of mouse L929 cells. Therefore, we conclude that the n-Ag/AD/PLGA dressing possesses good biocompatibility, antibacterial properties, and effectively release. It provides a new strategy for clinical treatment in diabetic foot infection.

Key words: Electrospinning, Dressing, Nano-silver, Poly(lactide-co-glycolide), Anisodamine

TrendMD:

CUI Weiwei, GAO Tianlin, LU Ying, ZHANG Peibiao, LIU Ya, LI Bo. Preparation and Biological Evaluation of Electrospun Nano-silver/Anisodamine/PLGA^†[J]. Chem. J. Chinese Universities, 2016, 37(1): 195.

Figures/Tables 7

Fig.1 Antibacterial test results against E. coli(A) and S.aureus(B) on n-Ag/PLGA fibers with different contents of n-Ag after 24 h incubation ^Mass fration of n-Ag(%): a. 0; b. 2; c. 4; d. 8.

Fig.2 ESEM images of n-Ag/AD/PLGA electrospun fibers(* P<0.05) ^Mass fraction of AD in n-Ag/AD/PLGA(%): (A) 0; (B) 1; (C) 5; (D) 10. (E) Average diameters of (A—D).

Fig.3 FTIR spectra of PLGA(a), n-Ag/PLGA(b) and n-Ag/AD/PLGA fibers(c)

Fig.4 Cumulative release curve of n-Ag/AD/PLGA fibers with different contents of AD^Mass fraction of AD(%): a.1; b. 5; c. 10.

Fig.5 Antibacterial test results against E. coli (A) and S. aureus(B) on fibers with different contents of AD after 24 h incubation and average diameters of inhibition ring(* P<0.05)(C)^ a. PLGA; mass fraction of AD(%) in n-Ag/AD/PLGA: b. 0, c. 1, d. 5, e. 10.

Fig.6 MTT test of the cytotoxicity of different electrospun fibers(* P<0.05) ^a. PLGA. Mass fraction of AD in n-Ag/AD/ PLGA(%): b. 0; c. 1; d. 5; e. 10.

Fig.7 Cell viability of L929 cells cultured on different electrospun fibers(* P<0.05)^ a. PLGA. Mass fraction of AD in n-Ag/AD/PLGA(%):b. 0; c. 1; d. 5; e. 10.

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