Microdroplet Detection of Protein Based on Superhydrophobic Polystyrene Film†

doi:10.7503/cjcu20180106

Abstract

Abstract:

Superhydrophobic polystyrene film with low water sliding angle was prepared by electrospinning, and the microdroplet detection technology of protein based on the superhydrophobic film was established. The superhydrophobic surface ensures the formation of spherical droplets, and the amount of sample required was thus decreased from hundreds of microliters to 10 μL. The low water sliding angle facilitates the manipulation of microdroplets and recycling of the detection substrates. Rapidly qualitative and sensitively quantitative detection of bovine serum albumin(BSA) molecules was achieved by the combination of biuret colorimetric assay and the microdroplet detection technology based on the superhydrophobic surface. Compared with the solution phase detection, the detection limit was decreased from 24.53 μmol/L to 1.490 μmol/L after drying the microdroplet on the electrospining polystyrene(PS) film due to the better concentration effect of the analytes on the superhydrophobic surface. This microdroplet detection technology of protein has a certain degree of universality. This study provides a new technical platform for the highly sensitive detection of trace biological/chemical molecules.

Key words: Electrospinning, Superhydrophobic polystyrene film, Low water sliding angle, Microdroplet detection of protein

CLC Number:

O656
O631

TrendMD:

XU Dan,DING Yadan,WANG Xue,CONG Tie,LIU Junping,HONG Xia,PAN Ying. Microdroplet Detection of Protein Based on Superhydrophobic Polystyrene Film^†[J]. Chem. J. Chinese Universities, 2018, 39(9): 1913.

Figures/Tables 7

Fig.1 SEM images of the electrospining PS film(A) and smooth PS film(B) Inset is the magnification of (A).

Fig.2 Water contact angle of the smooth PS film(A) and the electrospining PS film(B) Inset in (B) is water sliding angle of the electrospining PS film.

Fig.3 Reaction product structure of protein and biuret reagent(A), the microdroplet detection photographs of BSA before(B) and after(C) the droplet of biuret solution and the droplet of BSA solution coalesced with different concentrations of BSA(from left to right: 1.490, 14.90, 149.00 and 1490.00 μmol/L)(D) and UV-Vis spectra of BSA(a), biuret(b) and their reaction product(c)(E)

Fig.4 Absorption spectra of biuret upon the addition of various concentrations of BSA(A), the relationship between the integral area of absorption and the concentration of the BSA(B), the absorption spectra of Biuret upon the addition of various concentrations of BSA after drying on the electrospining PS film(C) and the relationship between the integral area of absorption and the concentration of the BSA after drying on the electrospining PS film(D)The insets are a linear relationship of absorption integral area versus the concentration of BSA over the range of 29.80—119.2 μmol/L(B) and a linear relationship of absorption integral area versus the concentration of BSA after drying on the electrospining PS film over the range from 1.490 μmol/ to 149.0 μmol/L(D), respectively.

Fig.5 Photographs of the dried reaction product on hydrophobic surface(A) and superhydrophobic surface(B)

Fig.6 Absorption spectra of biuret upon the addition of various concentrations of BSA after drying on smooth PS film(A) and the relationship between the integral area of absorption and the concentration of the BSA after drying on smooth PS film(B)The inset in (B) is a linear relationship of absorption integral area versus the concentration of BSA after drying on smooth PS film over the range from 74.50 μmol/L to 149.0 μmol/L.

Fig.7 Photographs of the microdroplet detection in biuret solution and LZM solution(A), biuret solution and milk powder solution(C) and their coalesced droplet on superhydrophobic surface(B, D)

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