Fabrication and Characterization of Alginate/Chitosan Polyelectrolyte Complex Elastic Scaffolds†

doi:10.7503/cjcu20141094

Abstract

Abstract:

Elastic scaffolds composed of sodium alginate(SA) and chitosan(CS) were fabricated via electrostatic interaction between —COOH, —NH₂ groups and phase separation technique. This study presented the influence of freezing temperature, solid content on pore size and molar ratio on mechanical strength, hydrophilicity, biodegradability and biocompatibility. The results showed that average pore size of alginate/chitosan scaffolds fabricated from 2%(mass fraction) solid content and at a freezing temperature of -24 ℃ was between 110 and 170 μm. High swelling ratio of more than 1400% showed the excellent hydrophilic performance of these scaffolds. Degradation of the alginate/chitosan scaffolds could be modified by varying the molar ratio(—COOH/—NH₂). The mechanical strength of the scaffolds could be controlled by different molar ratios and solid contents. The scaffolds exhibited good elastic behavior and fatigue durability by cyclic mechanical test in wet state. In vitro culture of rabbit adipose-derived stem cells(rASCs) indicated that aggregated rASCs were formed in the scaffolds when the molar ratios were 2:1 and 1:1, while adherent rASCs were found in the scaffolds with molar ratio of 1:2. Hence cellular adhesion behavior can be regulated by varying scaffold component.

Key words: Tissue engineering scaffold, Alginate, Chitosan

CLC Number:

O636

TrendMD:

ZHANG Weijun, ZHANG Kunxi, LI Guifei, ZHANG Danqing, YIN Jingbo. Fabrication and Characterization of Alginate/Chitosan Polyelectrolyte Complex Elastic Scaffolds^†[J]. Chem. J. Chinese Universities, 2015, 36(4): 758.

Figures/Tables 9

Fig.1 Fabricating process SA/CS scaffolds

Fig.2 FTIR spectra of scaffolds a. SA; b. CS; c. SA/CS; d. SA/CS crosslinked with Ca2+.

Fig.3 SEM images of SA/CS scaffolds (A) -7 ℃; (B) -24 ℃; (C) -80 ℃, SA/CS molar ratio: 1:1, solid content: 2%; (D) solid content: 1%; (E) solid content: 2%; (F) solid content: 3%, SA/CS molar ratio: 1:1, freezing temperature: -24 ℃.

Fig.4 Pore size distributions with different temperature(A) and different solid content(B) and porosity of SA/CS scaffolds(molar ratio 1:1) with different solid contents(C)

Fig.5 Elasticity presentation(A), compression modulus of SA/CS scaffolds(B, C) and cyclic mechanical test of SA/CS scaffolds(D—F) (B) SA/CS molar ratio 1:1; (C) solid content 2%; molar ratio of SA/CS: (D) 2:1; (E) 1:1; (F) 1:2.

Fig.6 Contact angle and swelling ratio of SA/CS scaffolds (A, C) Solid content: 2%; (B) SA/CS molar ratio: 1:1.

Fig.7 Degradation of SA/CS scaffolds(solid content 2%) with different molar ratios Molar ratio of SA/CS: a. 1:2; b. 1:1; c. 2:1.

Fig.8 Cell adhesion on SA/CS scaffolds for 7 d(solid content 2%) Molar ratio of SA/CS: (A) 2:1; (B) 1:1; (C, D) 1:2; (D) high magnification of (C).

Fig.9 DNA content of rASCs in SA/CS scaffolds for 7 d determined by Hoechst 33258 DNA assay(solid content 2%)

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