Chem. J. Chinese Universities ›› 2014, Vol. 35 ›› Issue (8): 1835.doi: 10.7503/cjcu20131236
• Polymer Chemistry • Previous Articles Next Articles
GUO Hua, YANG Chengling, WANG Wei, LAI Quanyong, YUAN Zhi*(
)
Received:2013-12-17
Online:2014-08-10
Published:2019-08-01
Contact:
YUAN Zhi
E-mail:zhiy@nankai.edu.cn
Supported by:CLC Number:
TrendMD:
GUO Hua, YANG Chengling, WANG Wei, LAI Quanyong, YUAN Zhi. Preparation of Liver-targeted Nano-prodrug Based on Sodium Alginate Derivative and the Study on Antitumor Activity†[J]. Chem. J. Chinese Universities, 2014, 35(8): 1835.
Fig.1 Structures of DOX-ALG-mOEG(A) and GA-ALG-mOEG(B) and schematic representation of DOX-ALG-mOEG/GA-ALG-mOEG NPs from DOX-ALG-mOEG and GA-ALG-mOEG(C)
Fig.2 1H NMR(A) and FTIR(B) spectra of ALG(a) and GA-ALG-mOEG(b)
Fig.3 CAC(A) and size distribution(B) of DOX-ALG-mOEG/GA-ALG-mOEG NPs Inset is TEM image of DOX-ALG-mOEG/GA-ALG-mOEG.
| Sample | Dh/nm | PDI* | Zeta potential/mV | CAC/(mg·L-1) |
|---|---|---|---|---|
| DOX-ALG/GA-ALG NPs | 253.5 | 0.123 | -44.4 | 58.34 |
| DOX-ALG-mOEG/GA-ALG-mOEG NPs | 254.2 | 0.207 | -35.1 | 5.52 |
Table 1 Characterization of DOX-ALG-mOEG/GA-ALG-mOEG NPs and GA-ALG/DOX-ALG NPs
| Sample | Dh/nm | PDI* | Zeta potential/mV | CAC/(mg·L-1) |
|---|---|---|---|---|
| DOX-ALG/GA-ALG NPs | 253.5 | 0.123 | -44.4 | 58.34 |
| DOX-ALG-mOEG/GA-ALG-mOEG NPs | 254.2 | 0.207 | -35.1 | 5.52 |
Fig.4 In vitro cumulative release of DOX-ALG-mOEG/GA-ALG-mOEG NPs(a, b) and DOX-ALG/GA-ALG NPs(c, d) in PBS under acidic(pH=4.0)(a, c) and neutral(pH=7.4)(b, d) condition
Fig.5 CLSM images of HepG2 cells after the treatment with DOX-ALG/GA-ALG NPs(A1—A3) and DOX-ALG-mOEG/GA-ALG-mOEG NPs(B1—B3) (A1, B1) DOX; (A2, B2) Hoechst; (A3, B3) Overlay.
Fig.6 CLSM images of HepG2 cells after treatment with DOX/GA-ALG NPs(A1—A3) and DOX/GA-ALG NPs+PEG-GA(B1—B3) (A1, B1) DOX; (A2, B2) Hoechst; (A3, B3) Overlay.
Fig.7 Cytotoxicity(A) and the IC50(B) of DOX-ALG-mOEG NPs, DOX-ALG/GA-ALG NPs and DOX-ALG-mOEG/GA-ALG-mOEG NPs against HepG2 cells[Mean±SD(n=3)]
Fig.8 Representative photographs(A) and ultimate tumor mass(B) of excised H22 tumors from mice treated with saline(a), DOX·HCl(b), DOX-ALG/GA-ALG NPs(c) and DOX-ALG-mOEG/GA-ALG-mOEG NPs(d)[means±SD(n=3—5), * P<0.05]
| [1] | Zuluaga M. F., Gabriel D., Lange N., Mol. Pharm., 2012, 9(6), 1570—1579 |
| [2] | She W. C., Luo K., Zhang C. Y., Wang G., Geng Y. Y., Li L., He B., Gu Z. W., Biomaterials, 2013, 34(5), 1613—1623 |
| [3] | Zhang J., Li C., Xue Z. Y., Cheng H. W., Huang F. W., Zhuo R. X., Zhang X. Z., Acta Biomater., 2011, 7(4), 1665—1673 |
| [4] | Liang H. Y., Wu X., Jin H. J., Chem. J. Chinese Universities, 2011, 32(6), 1389—1395 |
| (梁海岩, 吴学, 金慧娟. 高等学校化学学报, 2011, 32(6), 1389—1395) | |
| [5] | Zhang L., Hu C. H., Cheng S. X., Zhuo R. X., Colloids Surf. B Biointerfaces, 2010, 79(2), 427—433 |
| [6] | Guo M., Que C. L., Wang C. H., Liu X. Z., Yan H. S., Liu K. L., Biomaterials, 2011, 32(1), 185—194 |
| [7] | Huang W., Wang P., Wang W., Zhang Y., Zhang C. N., Tian Q., Wang X. H., Liu Y., Yuan Z., Chem. J. Chinese Universities, 2011, 32(2), 416—420(黄薇, 王平, 王蔚, 张玥, 张闯年, 田秦, 王秀华, 刘媛, 袁直. 高等学校化学学报, 2011, 32(2), 416—420) |
| [8] | Kim D., Lee E. S., Oh K. T., Gao Z. G., Bae Y. H., Small, 2008, 4(11), 2043—2050 |
| [9] | Tian Q., Wang X. H., Wang W., Zhang C. N., Wang P., Yuan Z., Nanomedicine, 2012, 8(6), 870—879 |
| [10] | Zhang C. N., Wang W., Liu T., Wu Y. K., Guo H., Wang P., Tian Q., Wang Y. M., Yuan Z., Biomaterials, 2012, 33(7), 2187—2196 |
| [11] | Tian Q., Zhang C. N., Wang X. H., Wang W., Huang W., Cha R. T., Wang C. H., Yuan Z., Liu M., Wan H. Y., Tang H., Biomaterials, 2010, 31(17), 4748—4756 |
| [12] | Wang Y., Zhang L., Li Y., Hou X., Zeng F., Carbohydr. Res., 2004, 339(15), 2567—2574 |
| [13] | Guo H., Yang C. L., Hu Z. P., Wang W., Wu Y. K., Lai Q. Y., Yuan Z., J. Mater. Chem. B, 2013, 43(1), 5933—5941 |
| [14] | Zhang C. N., Wang W., Wang C. H., Tian Q., Huang W., Yuan Z., Chen X. S., Science in China Series B: Chemistry, 2009, 52(9), 1382—1387 |
| [15] | Guo H., Lai Q., Wang W., Wu Y., Zhang C., Liu Y., Yuan Z., Int. J. Pharm., 2013, 451, 1—11 |
| [16] | Moradi E., Vllasaliu D., Garnett M., Falcone F., Stolnik S., RSC Advances, 2012, 2(7), 3025—3033 |
| [17] | Valencia P. M., Hanewich-Hollatz M. H., Gao W., Karim F., Langer R., Karnik R., Farokhzad O. C., Biomaterials, 2011, 32(26), 6226—6233 |
| [18] | Shen Y. Q., Jin E., Zhang B., Murphy C. J., Sui M., Zhao J., Wang J., Tang J., Fan M., van Kirk E., Murdoch W. J., J. Am. Chem. Soc., 2010, 132(12), 4259—4265 |
| [19] | Larsen E. K., Nielsen T., Wittenborn T., Birkedal H., Vorup-Jensen T., Jakobsen M. H., Ostergaard L., Horsman M. R., Besenbacher F., Howard K. A., Kjems J., ACS Nano, 2009, 3(7), 1947—1951 |
| [20] | Yuan H., Zhang S., Applied Physics Letters, 2010, 96(3), 033704-1—033704-3 |
| [21] | Fonge H., Huang H., Scollard D., Reilly R. M., Allen C., J. Control Release, 2012, 157(3), 366—374 |
| [22] | Kaneko T., Willner D., Monkovic I., Knipe J. O., Braslawsky G. R., Greenfield R. S., Vyas D. M., Bioconjugate Chemistry, 1991, 2(3), 133—141 |
| [23] | Huang W., Wang W., Wang P., Tian Q., Zhang C. N., Wang C. H., Yuan Z., Liu M., Wan H. Y., Tang H., Acta Biomater., 2010, 6(10), 3927—3935 |
| [24] | Dong L., Xia S., Wu K., Huang Z., Chen H., Chen J. N., Zhang J. F., Biomaterials, 2010, 31(24), 6309—6316 |
| [25] | Guo H., Yang C. L., Wang W., Wu Y. K., Lai Q. Y., Yuan Z., Chin. J. Polym. Sci., 2014, 32(5), 540—550 |
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