Chemical Journal of Chinese Universities ›› 2020, Vol. 41 ›› Issue (11): 2426-2434.doi: 10.7503/cjcu20209236

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Monitoring of Peroxynitrite Variation During Liver Injury Adopting a Far Red to Near-infrared Fluorescent Probe with Large Stokes Shift

WU Qian, CHENG Dan, LÜ Yun, YUAN Lin(), ZHANG Xiaobing   

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan University,Changsha 410082,China
  • Received:2020-07-18 Online:2020-11-10 Published:2020-11-06
  • Contact: YUAN Lin E-mail:lyuan@hnu.edu.cn
  • Supported by:
    This paper is supported by the National Natural Science Foundation of China(21877029);the China Postdoctoral Science Foundation(2018M642968)

Abstract:

Liver injury is one of the major problems affecting public health, and has attracted growing attention. It is universally believed that overexpression of peroxynitrite(ONOO?) plays an important role in the pathogenesis of liver injury and other diseases, and it is closely related to early liver injury. Therefore, in order to understand the role of ONOO? in the process of liver injury, an analysis method that can achieve high selectivity and real-time detection of ONOO? is urgently developed. Herein, we present a far red to near-infrared(FR-NIR) fluorescent probe with large Stokes shift to detect the ONOO? production in vivo during drug induced liver injury(DILI). Owing to the large Stokes shift, the FR-NIR probe(LSDQ-ONOO?) can not only reduce the interference of spectral overlap and self-absorption, but also improve the imaging signal-to-noise ratio. High sensitivity(detection limit=25.8 nmol/L) and excellent selectivity ensured that LSDQ-ONOO? can monitor subtle changes of ONOO?in vitro and in vivo during DILI.

Key words: Fluorescent probe, Large Stokes shift, Peroxynitrite, Drug induced liver injury

CLC Number: