高等学校化学学报

高等学校化学学报 ›› 1995, Vol. 16 ›› Issue (8): 1182.

• 论文 • 上一篇    下一篇

毛细管电泳-间接激光光热干涉检测方法研究

任吉存1, 李斌成1, 邓延倬2, 程介克1   

  1. 1. 武汉大学化学系, 武汉, 430072;
    2. 武汉大学分析测试科学系武汉, 430072
  • 收稿日期:1994-10-12 修回日期:1995-04-24 出版日期:1995-08-24 发布日期:1995-08-24
  • 通讯作者: 邓延倬
  • 作者简介:任吉存,男,38岁,副教授.现在湖南医科大学做博士后研究工作.
  • 基金资助:

    国家自然科学基金

Studies on Indirect Thermo-Optical Detection for Capillary Electrophoresis

REN Ji-Cun1, LI Bin-Cheng1, DENG Yan-Zhuo2, CHENG Jie-Ke1   

  1. 1. Department of Chemistry, Wuhan University, Wuhan, 430072;
    2. Department of Analysis and Testing Science, Wuhan University, Wuhan, 430072
  • Received:1994-10-12 Revised:1995-04-24 Online:1995-08-24 Published:1995-08-24

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被引次数

1

摘要： 本文提出毛细管电泳-间接激光光热干涉检测新方法。采用吸收系数大、吸收波长与泵浦激光(He-Ne)波长匹配性能较好的亚甲蓝溶液为背景电解质溶液,加入乙醇减少了毛细管壁对亚甲蓝的吸附作用。并将间接光热干涉检测法用于氨基酸毛细管电泳分离检测,对赖氨酸检测限达5×10-6mol/L(S/N=2).

关键词: 毛细管电泳, 间接光热检测, 氨基酸, 分离

Abstract: Indirect thermo-optical detection for capillary electrophoresis was presented first by combining indirect detection with thermo-optical method.In this detection,methylene blue solution was used as background electrolyte solution,because it has large molar absorption coefficient at 633 nm. This wavelength matches well with that of He-Ne laser(632.8 nm).The detection was applied to detect amino acids in capillary zone electrophoresis separation. The detection limit for lysine was 5×10-6mol/L(S/N=2).

Key words: Capillary electrophoresis, Indirect thermo-optical detection, Amino acid, Separation

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