高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (5): 737.

• 研究简报 • 上一篇    下一篇

分离尿激酶的胍基型亲和色谱填料研究

常建华1, 高俊萍1, 梁峰1, 冯越1, 董聿生1, 苏天升2, 郭立安3   

  1. 1. 西北大学化学系, 西安 710069;
    2. 中国科学院化学研究所, 北京 100080;
    3. 第四军医大学中心实验室, 西安 710032
  • 收稿日期:1999-07-13 出版日期:2000-05-24 发布日期:2000-05-24
  • 通讯作者: 常建华(1944年出生),男,教授,从事生物活性物质分离与精细有机化学品方面的研究.E-mail:changjh@163.net
  • 基金资助:

    陕西省教育委员会重点科研项目(批准号:96JZK15)

Studies on the Packings with Guanido of Affinity Chromatography for the Separation of Urokinase

CHANG Jian-Hua1, GAO Jun-Ping1, LIANG Feng1, FENG Yue1, DONG Yu-Sheng1, SU Tian-Sheng2, GUO Li-An3   

  1. 1. Department of Chemistry, Northwest University, Xian 710069, China;
    2. Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China;
    3. Central Laboratory, the Fourth Military University, Xi'an 710032, China
  • Received:1999-07-13 Online:2000-05-24 Published:2000-05-24

PDF (PC)

被引次数

7

关键词: 填料, 亲和色谱, 分离, 尿激酶

Abstract: Five guanido packings of affinity chromatography for the separation of urokinase have been synthesized. The separation characteristics and the effect of matrix and ligands on them have been studied. The guanidine derivative is a fine ligand, the packings with ligand of arginine are much better than that with ligand of p-ABZ. The polyepoxypropyl methacrylate microsphere is a good matrix. The comprehensive function of packings with ligand of arginine and matrix of polyepoxypropyl methacrylate microsphere is more excellent than Sepharose-p-ABZ.

Key words: Packings, Affinity chromatography, Separation, Urokinase

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