高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (10): 1770.

• 论文 • 上一篇    下一篇

SPR免疫竞争法研究小分子吗啡与其抗体间作用的动力学特征

陈受惠1,2, 崔小强1, 杨帆1, 倪永年2, 杨秀荣1   

  1. 1. 中国科学院长春应用化学研究所电分析化学国家重点实验室, 长春 130022;
    2. 南昌大学化学系, 南昌 330047
  • 收稿日期:2002-11-05 出版日期:2003-10-24 发布日期:2003-10-24
  • 通讯作者: 杨秀荣(1946年出生),女,博士,研究员,博士生导师,从事生物分析化学研究.E-mail:xryang@ns.ciac.jl.cn E-mail:xryang@ns.ciac.jl.cn
  • 基金资助:

    国家自然科学基金(批准号:20075027);国家重点基础研究规划项目(批准号:2001CB5102)资助

Kinetic Characteristics of the Interaction Between Morphine and Its Antibody by SPR Competitive Immunoassay

CHEN Shou-Hui1,2, CUI Xiao-Qiang1, YANG Fan1, NI Yong-Nian2, YANG Xiu-Rong1   

  1. 1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academyof Sciences, Changchun 130022, China;
    2. Department of Chemistry, Nanchang University, Nanchang 330047, China
  • Received:2002-11-05 Online:2003-10-24 Published:2003-10-24

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被引次数

17

摘要： 表面等离子体共振技术(SPR)主要应用于生物大分子相互作用的研究,本文采用溶液竞争法,测定了小分子吗啡与其抗体作用的结合常数K1,并计算了吗啡抗体与吗啡化牛血清白蛋白的结合常数K2.证明了多抗对抗原的亲和力较单抗大,并表明大分子蛋白质的存在对抗体与待测物的结合有阻碍作用.

关键词: 表面等离子体共振(SPR), 动力学, 吗啡, 溶液竞争法, 免疫分析

Abstract: It is impossible for surface plasmon resonance to measure directly the binding kinetics between a low-molecular-weight analyte interacting and its immobilized binding partner.Solution competition method was applied to the kinetic study of the interaction between morphine and its antibody.The affinity constant between the antibody of morphine and morphine-BSAimmobilized on the sensor chip was also obtained.The result showed that the affinity of polyclonal antibody is stronger than that of monoclonal antibody.And it also indicated that the protein combined with the analyte affected the binding of antibody to antigen.

Key words: Surface plasmon resonance(SPR), Kinetics, Morphine, Solution competition method, Immunoassay

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