高等学校化学学报

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (9): 1608.

• 研究论文 • 上一篇    下一篇

Nd(Ⅲ)与Hbbimp配合物的合成及其与DNA的作用研究

周春琼1, 邓先和1, 刘刚1, 杨频2   

  1. 1. 华南理工大学化工学院, 广州510640;
    2. 山西大学分子科学研究所, 太原030006
  • 收稿日期:2003-09-25 出版日期:2004-09-24 发布日期:2004-09-24
  • 基金资助:

    国家自然科学基金(批准号:20171031)资助

Synthesis of the Nd(Ⅲ) and Hbbimp Complex and Interaction Between the Complex and DNA

ZHOU Chun-Qiong1, DENG Xian-He1, LIU Gang1, YANG Pin2   

  1. 1. College of Chemical Engineering, South China University of Technology, Guangzhou 510640, China;
    2. Institute of Molecular Science, Shanxi University, Taiyuan 030006, China
  • Received:2003-09-25 Online:2004-09-24 Published:2004-09-24

PDF (PC)

被引次数

19

摘要： 合成并表征了新的双核配合物[Nd2(bbimp)(CH3COO)(CH3CH2O)2(CH3CH2OH)](ClO4)2,Hbbimp=2,6-二[二(2-苯并咪唑甲基)]氨甲基-4-甲基苯酚.用光谱学手段研究了配合物与小牛胸腺(CT)DNA的作用,结果表明,配合物使CT的DNA最大吸收峰发生减色和红移;使溴化乙锭(EB)-DNA复合物体系荧光强度减弱;热变性实验表明配合物使DNA的变性过程和降解过程共存.在50℃,pH=8.0时,单独的配合物对超螺旋质粒pBR322DNA的断裂的效果最好,可将大部分超螺旋DNA(CCC带)转化为缺刻产物(OC带);当c(H2O2)<1×10-3mol/L,n(配合物):n(H2O2)=1:20时,配合物在较低浓度时即可将CCC带全部转化为OC带.

关键词: 双核Nd(Ⅲ)配合物, Hbbimp, 合成, DNA

Abstract: A new complex [Nd2(bbimp)(CH3COO)(CH3CH2O)2(CH3CH2OH)](ClO4)2 was synthesized and characterized, in which Hbbimp is 2,6-bis[bis(2-benzimidazolylmethyl)]aminomethyl-4-methylphenoxo. The investigation on the interaction of both complex and CT DNA indicates that the absorbance value and the wave length at the maximum peak of CT DNA are reduced and red-shifted while the intensity of fluorescent spectra of EB-DNA is gradually weaken. UV melting curves prove the existence of the denaturation and degradation together. The only complex is capable of promoting the cleavage of pBR322 DNA at 50℃ and pH 8.0 and can translate most of the supercoiled DNA into nicked DNA. However, the supercoiled DNA can be absolutely translated into the nicked DNA when c(H2O2)<1×10-3 mol/L and n(complex):n(H2O2) =1:20.

Key words: Binuclear Nd(Ⅲ) complex, Hbbimp, Synthesis, DNA

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