高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (12): 2202.

• 论文 • 上一篇    下一篇

人脑枕叶区衰老进程的比较蛋白质组学研究

周波1, 杨伟1, 张景华2, 童元鹏1, 赵锐1, 纪建国1, 茹炳根1   

  1. 1. 北京大学生命科学学院蛋白质组学实验室, 北京 100871;
    2. 中国人民解放军总医院老年神经科, 北京 100853
  • 收稿日期:2002-11-04 出版日期:2003-12-24 发布日期:2003-12-24
  • 通讯作者: 纪建国(1962年出生),男,副教授,硕士生导师,主要从事蛋白质组学研究.E-mail:jijg@pku.edu.cn茹炳根(1936年出生),男,教授,博士生导师,主要从事蛋白质化学与蛋白质组学研究.E-mail:rulab@pku.edu.cn E-mail:rulab@pku.edu.cn
  • 基金资助:

    国家九五攻关项目(批准号:96-CO2-01-09);国家重点基础研究发展规划项目(批准号:001CB5102-07)资助

Comparative Proteome Analysis of Human Brain Occipital Lobes During the Course of Aging

ZHOU Bo1, YANG Wei1, ZHANG Jing-Hua2, TONG Yuan-Peng1, ZHAO Rui1, JI Jian-Guo+1, RU Bing-Gen1   

  1. 1. Laboratory of Proteome, College of Life Science, Peking University, Beijing 100871, China;
    2. Department of Geriatric Neurology, Chinese PLA General Hospital, Beijing 100853, China
  • Received:2002-11-04 Online:2003-12-24 Published:2003-12-24

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被引次数

27

摘要： 分别从23岁、64岁、72岁、83岁以及94岁无神经性和精神性疾病史个体大脑皮层枕叶区取样,制备蛋白质样品,进行双向凝胶电泳(2-DE)、考马斯亮蓝染色、凝胶扫描和ImageMaster 2DElite软件分析,每张胶上平均可检测到1000个以上蛋白质点.通过软件半定量分析,进一步研究了衰老过程中枕叶蛋白质的差异表达,发现随年龄增长有7种蛋白质有一致的显著上升或下降趋势.应用质谱进行肽质量指纹图谱(PMF)和/或肽序列标签(PST)分析,数据库检索共鉴定了11种蛋白质,其中有5种具有一致的上调或下调性,包括神经元突触结构蛋白低分子量神经丝蛋白(NeurofilamenttripletLprotein,NF-L)、参与抗氧化反应的硫氧还原蛋白过氧化物酶(Peroxiredoxin)、三羧酸循环关键酶(顺)乌头酸水合酶(Aconitatehydratase)和糖代谢途径中的关键酶烯醇化酶2(Enolase 2)以及分子伴侣蛋白T复合物蛋白1(T-complexprotein 1).首次建立了正常人脑枕叶区的双向电泳蛋白质表达图谱,针对人脑枕叶区蛋白质在衰老过程中的差异表达进行了研究,并对差异表达蛋白在衰老进程中可能的生物学意义做了探讨.

关键词: 蛋白质组, 双向凝胶电泳, 质谱, 人脑枕叶区, 衰老

Abstract: Proteomic analysis is a powerful tool to reveal the mechanisMOf functional changes of central nervous system and the origination, development and prognosis of central nervous disease. Human brain proteins were isolated from human occipital lobes at five ages from 23 to 94 years old, and separated by two dimensional gel electrophoresis. Over one thousand protein spots were resolved in one gel with commassie brilliant blue staining. Differential expression of proteins with increasing ages was analyzed by Image Master 2D Elite software processing. Seven protein spots were found to increase or decrease with increasing ages consistently. Taking advantage of the high sensitivity and accuracy of mass spectrometry, 11 proteins with significant changes were identified by peptide fingerprinting map(PMF) or peptide sequence tag(PST) obtained from MS or tandem MS(MS/MS). Among the identified proteins, the amount of 5 proteins were increased or decreased consistently, including neurofilament triplet Lprotein(NF-L), a protein involved in the maintenance of neuronal caliber; peroxiredoxin(EC 1.11.1.), an antioxidant enzyme; aconitate hydratase(EC 4.2.1.3), a key enzyme in tricarboxylic acid(TCA) cycle; enolase 2(EC 4.2.1.11), a key enzyme in glycolysis pathway; T-complex protein 1, a chaperone. In summary, the two dimensional map of human brain occipital lobe proteins was firstly established, significantly changed proteins during the course of aging were identified and their possible biological significance was discussed.

Key words: Proteome, 2D-PAGE, Mass spectrometry, Human brain occipital lobes, Aging

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