关键词: 成纤维细胞生长因子模拟肽, 甲基丙烯酰化羧甲基壳聚糖, 光固化水凝胶, 创面修复

Abstract: In this study, a fibroblast growth factor mimetic peptide(CFAP1) containing chitosan-binding domain(ChiBD) was first synthesized via the solid-phase synthesis method. Subsequently, CFAP1 was blended with carboxymethyl chitosan methacrylate(CCSMA) at an optimized ratio to fabricate a novel wound repair hydrogel through the photo-crosslinking approach. The results demonstrated that the CCSMA@CFAP1 hydrogel exerted a significant inhibitory effect on the growth of Escherichia coli(E.coli) and Staphylococcus aureus(S.aureus), and exhibited prominent antioxidant properties. Meanwhile, this hydrogel could remarkably enhance the adhesion and proliferation of NIH-3T3 fibroblasts, as well as upregulate the expression of COL-1 and VEGF in the cells. In vivo study indicated that CCSMA@CFAP1 accelerated wound healing by suppressing excessive inflammation, promoting tissue vascularization and tissue regeneration. Furthermore, the CCSMA@CFAP1 hydrogel possessed favorable biosafety profiles. Collectively, this research provides theoretical and experimental evidence for the application of related materials in wound repair.

Key words: Fibroblast growth factor mimetic peptide, Carboxymethyl chitosan methacrylate; Photo-crosslinked hydrogel, Wound healing