高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

芍药籽油乳液的构建及其对小鼠皮肤光损伤的修复作用

郭爽1，2，张硕1，2，韩櫂濂1，2，张梅3，朱鹤1，2，程志强1，2   

  1. 1. 吉林农业大学资源与环境学院化学专业 2. 吉林省特色资源健康产品与医用材料科技创新中心 3. 吉林农业大学植物保护学院 菌物学院应用化学专业
  • 收稿日期:2025-12-29 修回日期:2026-02-03 网络首发:2026-02-04 发布日期:2026-02-04
  • 通讯作者: 朱鹤 E-mail:zhuhe311@jlau.edu.cn
  • 基金资助:
    吉林省科技厅科技发展计划项目(批准号：20230204018YY)资助

Construction of Peony Seed Oil Emulsion and Its Repair Effect on Photodamage of Mouse Skin

GUO Shuang1.2, ZHANG Shuo1.2, HAN Zhaolian1.2, ZHANG Mei3, ZHU He1.2*, CHENG Zhiqiang1.2   

  1. 1. Department of Chemistry, College of Resources and Environment, Jilin Agricultural University 2. Jilin Provincial Innovation Centre for Health Products and Medical Materials from Local Resources 3. Department of Applied Chemistry, College of Plant Protection and Mycology, Jilin Agricultural University
  • Received:2025-12-29 Revised:2026-02-03 Online First:2026-02-04 Published:2026-02-04
  • Supported by:
    Supported by the Science and Technology Development Program of the Science and Technology Department of Jilin Province (No.20230204018YY)

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摘要： 通过建立UVB诱导的小鼠皮肤光损伤模型，系统评估了芍药籽油乳液(PSOE)的光损伤修复作用.本文采用Franz扩散池法比较PSOE与纯芍药籽油(PSO)的皮肤渗透与滞留性能；通过测定细胞内活性氧(ROS)水平评价其抗氧化能力；建立动物模型观察皮肤愈合情况、红斑程度及弹性变化，并开展组织病理学分析(HE与Masson染色)及分子检测(ELISA与免疫组化法检测TNF-α、IL-1β、IL-6、MMP-1表达).实验结果表明，PSOE的皮肤累积渗透量较PSO提升约1.5倍，皮肤滞留率提高约4倍；其清除UVB诱导ROS的能力显著优于市售乳液(CE)及空白乳液基质(EB).动物实验表明，PSOE处理组创面愈合率在第7天达92.31±3.53%，真皮层炎症细胞浸润减少，表皮厚度恢复近正常水平，胶原纤维密度增加.分子检测结果表明，PSOE使TNF-α、IL-1β、IL-6及MMP-1的表达下调.综上，PSOE通过增强透皮递送、抑制氧化应激、减轻炎症反应、促进胶原再生及调节基质代谢等多途径协同作用，实现对UVB诱导皮肤光损伤的有效修复.

关键词: 芍药籽油, 乳液, 光损伤, 胶原沉积, 炎症因子

Abstract: By establishing a UVB-induced mouse skin photodamage model, the photodamage repairing effect of peony seed oil emulsion (PSOE) was systematically evaluated. In this paper, the Franz diffusion cell method was used to compare the skin penetration and retention properties of PSOE and pure peony seed oil (PSO). The antioxidant capacity was evaluated by measuring the intracellular reactive oxygen species (ROS) level. An animal model was established to observe skin healing, erythema degree, and elasticity changes, and histopathological analysis (HE and Masson staining) and molecular detection (ELISA and immunohistochemistry to detect the expression of TNF-α, IL-1β, IL-6, and MMP-1) were carried out. The experimental results showed that the cumulative skin penetration amount of PSOE was approximately 1.5 times higher than that of PSO, and the skin retention rate increased by about 4 times. Its ability to scavenge UVB-induced ROS was significantly better than that of the commercial emulsion (CE) and the blank emulsion base (EB). Animal experiments indicated that the wound healing rate of the PSOE treated group reached 92.31±3.53% on the 7th day. The infiltration of inflammatory cells in the dermis decreased, the epidermal thickness recovered to nearly the normal level, and the density of collagen fibers increased. Molecular detection results showed that PSOE down-regulated the expression of TNF-α, IL-1β, IL-6, and MMP-1. In conclusion, PSOE effectively repairs UVB-induced skin photodamage through multiple synergistic pathways, including enhancing transdermal delivery, inhibiting oxidative stress, reducing the inflammatory response, promoting collagen regeneration, and regulating matrix metabolism.

Key words: peony seed oil, emulsion, photodamage, collagen deposition, inflammatory response

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