高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

负载新型多肽CFAP1的水凝胶敷料在伤口修复中的应用

汪德州,高愉淇,韩春雨,杨爽,宋文植   

  1. 吉林大学中日联谊医院口腔科
  • 收稿日期:2026-01-08 修回日期:2026-03-31 网络首发:2026-04-03 发布日期:2026-04-03
  • 通讯作者: 宋文植
  • 基金资助:
    吉林省自然科学基金项目(批准号：YDZJ202501ZYTS314)资助

Application of Hydrogel Dressings Loaded with a Novel Polypeptide CFAP1 in Wound Repair

WANG Dezhou, GAO Yuqi, HAN Chunyu, YANG Shuang, SONG Wenzhi*#br#   

  1. Dept.of Stomatology,China-Japan Union Hospital,Jilin university
  • Received:2026-01-08 Revised:2026-03-31 Online First:2026-04-03 Published:2026-04-03
  • Supported by:
    Supported by the Project of Jilin Provincial Natural Science Foundation，China(No.YDZJ202501ZYTS314)

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摘要： 首先通过固相合成的方法制备了含有壳聚糖特异性结合结构域(ChiBD)的成纤维细胞生长因子模拟肽(CFAP1)；然后将CFAP1与甲基丙烯酰化羧甲基壳聚糖(CCSMA)按优化的比例共混，通过光固化的方法制备新型创面修复水凝胶。研究结果表明，CCSMA@CFAP1水凝胶对大肠杆菌(E.coli)和金黄色葡萄球菌(S.aureus)的生长有明显的抑制作用，并且具有突出的抗氧化特性。同时，CCSMA@CFAP1水凝胶能够显著地促进NIH-3T3成纤维细胞的黏附与增殖，并上调细胞中COL-1和VEGF的表达。动物实验结果表明，CCSMA@CFAP1能够通过抑制过度炎症、促进组织血管化和组织再生，进而促进创面修复。同时，CCSMA@CFAP1水凝胶具备良好的生物安全性。本文研究结果为相关材料在创面修复方面的应用提供理论和实践数据。

关键词: 成纤维细胞生长因子模拟肽, 甲基丙烯酰化羧甲基壳聚糖, 光固化水凝胶, 创面修复

Abstract: In this study, a fibroblast growth factor mimetic peptide(CFAP1) containing chitosan-binding domain(ChiBD) was first synthesized via the solid-phase synthesis method. Subsequently, CFAP1 was blended with carboxymethyl chitosan methacrylate(CCSMA) at an optimized ratio to fabricate a novel wound repair hydrogel through the photo-crosslinking approach. The results demonstrated that the CCSMA@CFAP1 hydrogel exerted a significant inhibitory effect on the growth of Escherichia coli(E.coli) and Staphylococcus aureus(S.aureus), and exhibited prominent antioxidant properties. Meanwhile, this hydrogel could remarkably enhance the adhesion and proliferation of NIH-3T3 fibroblasts, as well as upregulate the expression of COL-1 and VEGF in the cells. In vivo study indicated that CCSMA@CFAP1 accelerated wound healing by suppressing excessive inflammation, promoting tissue vascularization and tissue regeneration. Furthermore, the CCSMA@CFAP1 hydrogel possessed favorable biosafety profiles. Collectively, this research provides theoretical and experimental evidence for the application of related materials in wound repair.

Key words: Fibroblast growth factor mimetic peptide, Carboxymethyl chitosan methacrylate; Photo-crosslinked hydrogel, Wound healing

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