荧光纳米探针用于细胞器pH检测的研究进展

doi:10.7503/cjcu20200279

高等学校化学学报 ›› 2020, Vol. 41 ›› Issue (9): 1933.doi: 10.7503/cjcu20200279

• 庆祝《高等学校化学学报》复刊40周年专栏 • 上一篇下一篇

荧光纳米探针用于细胞器pH检测的研究进展

公少华, 张霞, 李娜(), 唐波

山东师范大学化学化工与材料科学学院, 分子与纳米探针教育部重点实验室, 化学成像功能探针山东省高等学校协同创新中心, 山东师范大学分子与纳米科学研究院, 济南 250014

收稿日期:2020-05-21 出版日期:2020-09-10 发布日期:2020-09-02
通讯作者: 李娜 E-mail:lina@sdnu.edu.cn
基金资助:
国家重点研发计划项目(2019YFA0210100);国家自然科学基金（批准号(91753111);山东省重点研发计划项目(2018YFJH0502);山东省高等学校青年创新科技项目(2019KJC022)

Recent Progress of Fluorescent Nanoprobes for Organelle pH Detection

GONG Shaohua, ZHANG Xia, LI Na(), TANG Bo

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan 250014, China

Received:2020-05-21 Online:2020-09-10 Published:2020-09-02
Contact: LI Na E-mail:lina@sdnu.edu.cn
Supported by:
Supported by the National Key Research and Development Program of China(2019YFA0210100);the National Natural Science Foundation of China(91753111);he Key Research and Development Program of Shandong Province, China(2018YFJH0502);the Youth Innovation Science and Technology Program of Higher Education Institution of Shandong Province, China(2019KJC022)

摘要/Abstract

摘要：

pH稳态对于维持活细胞细胞器的正常功能具有重要作用. 细胞器内pH稳态被打破会导致细胞器功能的紊乱, 进而引发癌症、神经退行性疾病等相关疾病. 因此, 在活细胞水平上定量测定pH并对其波动进行实时监测对于理解相关疾病的发生机制非常重要. 基于非侵入、高时空分辨率成像的优势, 荧光探针非常适合用于活细胞内pH的检测. 本综述总结了近些年利用不同种类荧光纳米探针对不同细胞器进行pH成像的研究工作, 并对荧光纳米探针应用面临的机遇与挑战进行了展望.

关键词: 荧光纳米探针, 细胞器, pH, 检测, 成像

Abstract:

pH homeostasis plays a significant role in maintaining the normal functions of different organelles in living cells. The disruption of pH homeostasis will result in the disorder of organelle function and further lead to the relevant diseases, such as cancer, neurodegenerative disease, etc. Therefore, it is important to quantitively detect pH and monitor the fluctuation of organelle pH in living cells to explore the pathology of relevant diseases. Fluorescent probes are suitable for pH detection in living cell based on the advantages of noninvasive and high spatiotemporal resolution imaging. In this review, we summarized the recently developed fluorescent nanoprobes for the pH detection of different organelles. The chances and challenges of the practical application of fluorescent nanoprobes were also discussed.

Key words: Fluorescent nanoprobe, Organelles, pH, Detection, Imaging

中图分类号:

O657

TrendMD:

公少华, 张霞, 李娜, 唐波. 荧光纳米探针用于细胞器pH检测的研究进展. 高等学校化学学报, 2020, 41(9): 1933.

GONG Shaohua, ZHANG Xia, LI Na, TANG Bo. Recent Progress of Fluorescent Nanoprobes for Organelle pH Detection. Chem. J. Chinese Universities, 2020, 41(9): 1933.

图/表 9

Fig.7 Design of dual?modified SiNPs(DMSiNPs)?based pH nanoprobe[66](A) Schematic of the synthesis of DMSiNPs; (B) images of SiNPs(1), DA(2), RBITC(3), and DMSiNPs(4) in ambient environment and under UV irradiation(λex=360 nm) as well as TEM image of DMSiNPs; (C) illustration of cellular endocytosis of DMSiNPs. Inset of (B) presents the enlarged HRTEM image of a single DMSiNPs; inset of (C) shows charge?transfer mechanism of DMSiNPs at acidic or basic conditions. Copyright 2016, American Chemical Society.

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荧光纳米探针用于细胞器pH检测的研究进展

Recent Progress of Fluorescent Nanoprobes for Organelle pH Detection

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