黄芪药渣中阿拉伯木聚糖(AX-Ⅰ-1)的提取纯化、 结构分析及体外抗氧化作用

doi:10.7503/cjcu20160500

高等学校化学学报 ›› 2016, Vol. 37 ›› Issue (12): 2168.doi: 10.7503/cjcu20160500

黄芪药渣中阿拉伯木聚糖(AX-Ⅰ-1)的提取纯化、结构分析及体外抗氧化作用

刘磊^1,^2,³, 李科^2,³(), 郝霞^1,^2,³, 王桂臻^1,^2,³, 秦雪梅^2,³, 杜冠华⁴, 张翔⁵

1. 山西大学化学化工学院, 2. 中医药现代研究中心, 太原 030006
3. 中药功效物质与新药创制山西省重点实验室, 太原 030006
4. 中国医学科学院、中国协和医科大学药物研究所, 北京 100050
5. 美国路易斯维尔大学, 路易斯维尔 40201

收稿日期:2016-07-13 出版日期:2016-12-10 发布日期:2016-11-11
作者简介:联系人简介: 李科, 男, 博士, 副教授, 主要从事糖化学与糖生物学方面的研究. E-mail:like@sxu.edu.cn
基金资助:
国家自然科学基金(批准号: 31300278)、山西省科技攻关计划(批准号: 20150313004-5)和教育部博士点新教师类基金(20131401120006)资助

Extraction, Separation, Structural Analysis and Antioxidant Activity in vitro of Arabinoxylans(AX-Ⅰ-1) from the Residue of Astragalus Root^†

LIU Lei^1,^2,³, LI Ke^2,^3,^*(), HAO Xia^1,^2,³, WANG Guizhen^1,^2,³, QIN Xuemei^2,³, DU Guanhua⁴, ZHANG Xiang⁵

1. College of Chemistry and Chemical Engineering
2. Modern Research Center for Traditional Chinese Medicine,Shanxi University, Taiyuan 030006, China
3. The Efficacy of Chinese Medicine Material and New Drug Key Lab of Shanxi Province, Taiyuan 030006, China
4. Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China
5. Medical College, University of Louisville, Louisville 40201, USA

Received:2016-07-13 Online:2016-12-10 Published:2016-11-11
Contact: LI Ke E-mail:like@sxu.edu.cn
Supported by:
† Supported by the National Natural Science Foundation of China(No.31300278), the Science and Technology Research Program Fund for Shanxi Province, China(No.20150313004-5) and the Ministry of Education PhD New Teacher Fund, China(No.20131401120006)

摘要/Abstract

摘要：

黄芪药渣经分级提取, 再通过二乙氨乙基(DEAE)-纤维素52阴离子交换柱层析和Sephacryl S-400 HR凝胶柱层析分离纯化, 得到4个多糖组分AX-Ⅰ-1~AX-Ⅰ-4. 对多糖AX-Ⅰ-1的理化性质和结构进行研究发现, AX-Ⅰ-1含有鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖(摩尔比为0.006∶14.113∶8.284∶0.116∶0.468∶1), 主链由阿拉伯糖和木糖通过β-(1→2), (1→3)和(1→4)苷键组成, 支链由(1→4)βArap, (1→3)βGalp和(1→2)βMan组成, 非还原末端由αRhap, βGclp和βGalp组成. 对多糖AX-Ⅰ-1~AX-Ⅰ-4的抗氧化研究结果表明, 这4个组分对羟基自由基、超氧阴离子自由基和1,1-二苯基-2-三硝基苯肼(DPPH)自由基均有一定的清除作用; 当浓度为0.1 mg/mL时, 多糖AX-Ⅰ-2~AX-Ⅰ-4对超氧阴离子的清除率是阳性对照维生素C(Vc)的2倍.

关键词: 黄芪药渣, 阿拉伯木聚糖, 分离纯化, 结构分析, 抗氧化

Abstract:

Four fractions(AX-Ⅰ-1, AX-Ⅰ-2, AX-Ⅰ-3 and AX-Ⅰ-4)from the residue of Astragalus root were extracted by sequential chemical extraction, and purified by DEAE-Cellulose 52 anion-exchange and Sepharcryl S-400 high resolution gel-permeation chromatography. A combination of chemical and instrumental analysis was performed to investigate the structural characterization and antioxidant activity of AX-Ⅰ-1. The results demonstrated that AX-Ⅰ-1 was mainly composed of rhamnose, arabinose, xylose, mannose, glucose and galactose, and the molar ratio was 0.006∶14.113∶8.284∶0.116∶0.468∶1. Backbone of AX-Ⅰ-1 composed of arabinose and xylose by β-(1→2), β-(1→3)and β-(1→4)glycosidic bond. The branches were composed of (1→4)-linked-βArap, (1→3)-linked-βGalp, (1→2)-linked-βManp, and the terminal residues were αRhap, βGclp, βGalp.The four fractions had a certain scavenging effect on hydroxyl radical, superoxide anion radical and DPPH radical. When the concentration is 0.1 mg/mL, scavenging rate of AX-Ⅰ-2, AX-Ⅰ-3 and AX-Ⅰ-4 on superoxide anion is about 2 times higher than the positive control vitamin C.

Key words: Residue of astragalus root, Arabinoxylan, Purification, Structural analysis, Antioxidant activity

TrendMD:

刘磊, 李科, 郝霞, 王桂臻, 秦雪梅, 杜冠华, 张翔. 黄芪药渣中阿拉伯木聚糖(AX-Ⅰ-1)的提取纯化、结构分析及体外抗氧化作用. 高等学校化学学报, 2016, 37(12): 2168.

LIU Lei, LI Ke, HAO Xia, WANG Guizhen, QIN Xuemei, DU Guanhua, ZHANG Xiang. Extraction, Separation, Structural Analysis and Antioxidant Activity in vitro of Arabinoxylans(AX-Ⅰ-1) from the Residue of Astragalus Root^†. Chem. J. Chinese Universities, 2016, 37(12): 2168.

图/表 9

参考文献 30

[1]	Gu G.G., Sheng Nong’s Herbal Classi, People’s Medical Publishing House, Bejing, 1995, 11—15
	(顾观光. 神农本草经, 北京: 人民卫生出版社, 1995, 11—15)
[2]	Pan H. F., Deng Q. D., Zhao Z. M., Lishizhen Medicine and Materia Medica Research, 2011, 22(8), 2026—2027
	(潘华峰, 邓乔丹, 赵自明. 时珍国医国药,2011, 22(8), 2026—2027)
[3]	Jiang M. H., Zhu L., Jiang J. G., Expert Opinion, 2010, 14(12), 367—1402
[4]	Zhou S. M., Liu X. Z., Guo Y., Carbohydr. Polym., 2010, 81, 784—789
[5]	Zhou Y., Li S., Qian Q., Liu X., Plant J., 2009, 57, 446—462
[6]	Cao L., Liu X.Z., Qian T. X., Sun G. B., Guo Y., Chang F. J., Zhou S. M., Sun X. B., Inter. J. Biol. Macromol., 2011, 48, 160—164
[7]	Hao X., Li K., Wang G. Z., Journal of Shanxi Medical University, 2016, 47(4), 338—343
	(郝霞, 李科, 王桂臻. 山西医科大学学报,2016, 47(4), 338—343)
[8]	Zhou Y. H., Li S. B., Qian Q., Plant J., 2009, 57, 446—462
[9]	Sun J. X., Sun X. F., Sun R. C., Carbohydrate Polymers, 2004, 56, 195—199
[10]	Cheng X. Q., Li K., Chen X. M., Journal of Beijing Forestry University, 2012, 34(5), 44—49
[11]	An X. J., Feng L., Song H. P., Journal of Biology, 2012, 29(3), 39—41
	(安晓娟, 冯琳, 宋红平. 生物学杂志,2012, 29(3), 39—41)
[12]	Liu Y., Yin L., Gong G. P., Peng Y. F., Huang L. J., Wang Z. F., Chem. J. Chinese Universities, 2016, 37(2), 261—268
	(刘洋, 殷璐, 龚桂萍, 彭艺芳, 黄琳娟, 王仲孚. 高等学校化学学报,2016, 37(2), 261—268)
[13]	Mouna S., Hager L., Mohamed D., Santiageo G., Chem. Res. Chinese Universities, 2015, 31(1), 16—20
[14]	Gao F. R., Li K., Hao X., Chinese Traditional and Herbal Drugs, 2015, 46(14), 2134—2142
	(高凡茸, 李科, 郝霞. 中草药,2015, 46(14), 2134—2142)
[15]	Ciucan I., Kerek F., Carbohydr. Res., 1984, 131(2), 209—217
[16]	Tang X. B., Li Z. H., Li Y. H., Liu W., Yu P., Li L. X., Guo Y., Chem. Res. Chinese Universities, 2015, 31(1), 71—77
[17]	Ma J. B., Shen Y. S., Li S., Food Chemistry, 2008, 27, 380—382
[18]	Gregory P., Food Chemistry, 2015, 169, 430—438
[19]	Sheng J. W., Sun Y. L., Carbohydrate Polymers, 2014, 108, 41—45
[20]	Chaikumpollert O., Methacanon P., Suchiva K., Carbohydrate Polymers, 2004, 57(2), 191—196
[21]	Roy A. K., Sen S. K., Bag S. C., Journal of Applied Polymer Science, 1991, 42(11), 2943—2950
[22]	Sun R. C., Sun X. F., Carbohydrate Polymers, 2002, 49(4), 415—423
[23]	Sun R. C., Lawther J. M., Banks W. B., Carbohydrate Polymers, 1996, 29(4), 325—331
[24]	Pu X. Y., Ma X. L., Liu L., Carbohydrate Polymers, 2016, 137, 154—164
[25]	Leone S., Molinaro A., Carbohydrate Research, 2007, 342, 1514—1518
[26]	Ji Y. H., Zhang M. S., Xu L., Journal of Changchun University of Traditional Chinese Medicine, 1999, 15(3), 62—63
	(纪耀华, 张明淑, 徐力. 长春中医学院学报,1999, 15(3), 62—63)
[27]	Wang Y., Zhao Y. M., Zhang Q. F., Chinese Traditional and Herbal Drugs, 2001, 32(11), 962—964
	(王莹, 赵毅民, 张起凤. 中草药,2001, 32(11), 962—964)
[28]	Fang S. D., Xu X. Y., Ye C. Q., Chinese J. Org. Chem., 1982, 2, 26—31
	(方圣鼎, 徐小异, 叶纯渠.有机化学, 1982, 2, 26—31)
[29]	Chen Q. R., Lin Z. H., Ding L. X., Chinese Journal of Modern Applied Pharmacy, 1985, 2(6), 11—13
	(陈启荣, 林振华, 丁禄霞. 中国现代应用药学,1985, 2(6), 1113—1115)
[30]	Ghoneum M., Gollapudi S., Cancer Lett., 2013, 10(201), 41—49

Sugar	Methylated sugar	m/z	Molar ratio	Linkages type
Rha-	2,3,4-Me₃-Rha	59, 72, 85, 102, 113, 118, 131, 162, 175	0.01	L-Rhap-(1→
Ara-	2-Me-Ara	59, 74, 85, 99, 118, 127, 141, 159, 173, 187, 201, 217, 261	13.42	→3,4)-L-Arap-(1→
Xyl-	2-Me-Xyl	61, 73, 86, 115, 128, 145, 159, 175, 188, 218, 243, 261, 289	8.13	→2,3,4)-D-Xylp-(1→
Man-	3,4,6-Me₃-Man	60, 71, 87, 99, 117, 129, 141, 161, 171, 189	0.10	→2)-D-Manp-(1→
Glc-	2,3,4,6-Me₄-Glc	59, 71, 87, 102, 118, 129, 145, 162, 175, 205	0.24	D-Glcp-(1→
	4-Me-Glc	59, 71, 87, 100, 116, 129, 143, 160, 171, 189, 201, 262	0.23	→2,3,6)-D-Glcp-(1→
Gal-	4,6-Me₂-Gal	59, 71, 86, 101, 112, 129, 143, 161, 188, 202	0.48	→2,3)-D-Galp-(1→
	3,4,6-Me₃-Gal	59, 71, 88, 100, 113, 119, 129, 145, 161, 174, 190	0.49	→2)-D-Galp-(1→

Sugar	Methylated sugar	m/z	Molar ratio	Linkages type
Rha-	2,3,4-Me₃-Rha	59, 72, 85, 102, 113, 118, 131, 162, 175	0.01	L-Rhap-(1→
Ara-	2-Me-Ara	59, 74, 85, 99, 118, 127, 141, 159, 173, 187, 201, 217, 261	13.42	→3,4)-L-Arap-(1→
Xyl-	2-Me-Xyl	61, 73, 86, 115, 128, 145, 159, 175, 188, 218, 243, 261, 289	8.13	→2,3,4)-D-Xylp-(1→
Man-	3,4,6-Me₃-Man	60, 71, 87, 99, 117, 129, 141, 161, 171, 189	0.10	→2)-D-Manp-(1→
Glc-	2,3,4,6-Me₄-Glc	59, 71, 87, 102, 118, 129, 145, 162, 175, 205	0.24	D-Glcp-(1→
	4-Me-Glc	59, 71, 87, 100, 116, 129, 143, 160, 171, 189, 201, 262	0.23	→2,3,6)-D-Glcp-(1→
Gal-	4,6-Me₂-Gal	59, 71, 86, 101, 112, 129, 143, 161, 188, 202	0.48	→2,3)-D-Galp-(1→
	3,4,6-Me₃-Gal	59, 71, 88, 100, 113, 119, 129, 145, 161, 174, 190	0.49	→2)-D-Galp-(1→

Residue	δ
Residue	1-H/C1	2-H/C2	3-H/C3	4-H/C4	5-H/C5	6-H/C6
α-L-Rhap(1→ (A)	5.02/102.4	4.34/70.9	3.99/81.0	3.44/72.0	3.83/69.5	1.24/
→3,4)β-L-Arap(1→(B)	4.26/102.2	4.22/75.9	3.87/83.8	3.99/82.1	3.82/63.7	-/-
→2,3,4)β-D-Xylp(1→(C)	4.76/107.0	3.07/74.4	3.44/73.1	3.82/81.6	3.36/59.6	-/-
→2)β-D-Manp(1→(D)	4.91/94.6	3.36/80.9	3.25/75.9	3.87/73.1	3.82/70.0	3.71/63.7
β-D-Glcp(1→ (E)	4.87/99.6	3.44/73.1	3.82/74.4	3.66/66.9	3.71/70.1	3.72/59.6
→2,3,6)α-D-Glcp(1→(F)	5.01/99.8	3.42/81.0	3.66/73.1	3.71/75.4	3.82/70.0	3.87/73.1
→2,3)β-D-Galp(1→(G)	4.84/102.4	3.82/69.3	3.99/82.6	4.04/70.0	3.66/75.9	3.71/59.6
→2)β-D-Galp(1→(H)	4.82/102.0	3.72/70.0	3.87/73.6	3.89/71.8	3.44/74.8	3.72/60.5

Residue	δ
Residue	1-H/C1	2-H/C2	3-H/C3	4-H/C4	5-H/C5	6-H/C6
α-L-Rhap(1→ (A)	5.02/102.4	4.34/70.9	3.99/81.0	3.44/72.0	3.83/69.5	1.24/
→3,4)β-L-Arap(1→(B)	4.26/102.2	4.22/75.9	3.87/83.8	3.99/82.1	3.82/63.7	-/-
→2,3,4)β-D-Xylp(1→(C)	4.76/107.0	3.07/74.4	3.44/73.1	3.82/81.6	3.36/59.6	-/-
→2)β-D-Manp(1→(D)	4.91/94.6	3.36/80.9	3.25/75.9	3.87/73.1	3.82/70.0	3.71/63.7
β-D-Glcp(1→ (E)	4.87/99.6	3.44/73.1	3.82/74.4	3.66/66.9	3.71/70.1	3.72/59.6
→2,3,6)α-D-Glcp(1→(F)	5.01/99.8	3.42/81.0	3.66/73.1	3.71/75.4	3.82/70.0	3.87/73.1
→2,3)β-D-Galp(1→(G)	4.84/102.4	3.82/69.3	3.99/82.6	4.04/70.0	3.66/75.9	3.71/59.6
→2)β-D-Galp(1→(H)	4.82/102.0	3.72/70.0	3.87/73.6	3.89/71.8	3.44/74.8	3.72/60.5

黄芪药渣中阿拉伯木聚糖(AX-Ⅰ-1)的提取纯化、结构分析及体外抗氧化作用

Extraction, Separation, Structural Analysis and Antioxidant Activity in vitro of Arabinoxylans(AX-Ⅰ-1) from the Residue of Astragalus Root^†

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 30

相关文章 15

编辑推荐

Metrics

本文评价

[1]	黄罗仪, 翁约约, 黄旭慧, 王朝杰. 车前草中黄酮类成分结构和性质的理论研究[J]. 高等学校化学学报, 2021, 42(9): 2752.
[2]	胡皓程, 李文利, 张嘉宁, 刘宇博. 黑木耳寡糖的提取、结构表征及生物活性[J]. 高等学校化学学报, 2021, 42(8): 2465.
[3]	杨依然, 姚华, 闫江红, 孙志恒, 张余, 房雪晴, 李绪文, 金永日. 薤中新的甾体皂苷类化学成分[J]. 高等学校化学学报, 2021, 42(6): 1742.
[4]	帅蝶, 赵美娟, 陈丙年, 王力. 4种Keggin型磷钼酸盐对蘑菇酪氨酸酶活性和黑色素生成的抑制及抗氧化作用[J]. 高等学校化学学报, 2021, 42(12): 3579.
[5]	韩方杰, 代梦娇, 梁芷珊, 宋忠乾, 韩冬雪, 牛利. 光电化学技术应用于抗氧化分析的研究进展[J]. 高等学校化学学报, 2020, 41(4): 591.
[6]	袁中文, 贺利贞, 陈填烽. 单原子催化剂的生物医学应用[J]. 高等学校化学学报, 2020, 41(12): 2690.
[7]	李翠勤, 翟雪, 孙鹏, 高宇新, 张志秋, 王俊, 李锋. 树状桥联半受阻酚类抗氧剂的合成与抗氧化性能[J]. 高等学校化学学报, 2019, 40(7): 1535.
[8]	金鑫, 嵇国利, 赵小亮, 李国云, 于广利. 一种刺松藻来源的硫酸阿拉伯聚糖的制备及特征结构表征[J]. 高等学校化学学报, 2019, 40(4): 733.
[9]	梁芷珊, 倪爽, 代梦娇, 韩方杰, 韩立鹏, 牛利, 韩冬雪. 基于g-C₃N₄/P25复合光电敏感体系的中草药抗氧化容量测定[J]. 高等学校化学学报, 2019, 40(10): 2081.
[10]	蒋静, 黄亚励, 张奇龙, 徐红, 孙晓红. 八元瓜环对氯化矢车菊素溶解性、稳定性及抗氧化性的影响[J]. 高等学校化学学报, 2019, 40(1): 76.
[11]	陈军超, 姚远, 张辉. 氮氧化物TEMPO清除过氧自由基的机理研究[J]. 高等学校化学学报, 2018, 39(9): 1961.
[12]	王雁冰, 张辉, 杨峻山, 陈炳鹏, 孙佳明. 破骨风中木脂素类化合物的分离鉴定及抗氧化活性[J]. 高等学校化学学报, 2018, 39(9): 1942.
[13]	张岩, 张盛明, 方桂珍. Ce掺杂固体酸活化的碱木质素的抗氧化性能[J]. 高等学校化学学报, 2018, 39(6): 1255.
[14]	高云霄, 胡小龙, 王月荣, 姜志宏, 章弘扬, 张敏, 胡坪. 铁皮石斛多糖的初级结构分析[J]. 高等学校化学学报, 2018, 39(5): 934.
[15]	曹玉惠, 张娟娟, 王再扬, 赵元晖. 牡蛎源类蛋白反应修饰肽的分离纯化及肽锌螯合物的结构表征[J]. 高等学校化学学报, 2018, 39(3): 470.

黄芪药渣中阿拉伯木聚糖(AX-Ⅰ-1)的提取纯化、 结构分析及体外抗氧化作用

Extraction, Separation, Structural Analysis and Antioxidant Activity in vitro of Arabinoxylans(AX-Ⅰ-1) from the Residue of Astragalus Root†

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 30

相关文章 15

编辑推荐

Metrics

本文评价

黄芪药渣中阿拉伯木聚糖(AX-Ⅰ-1)的提取纯化、结构分析及体外抗氧化作用

Extraction, Separation, Structural Analysis and Antioxidant Activity in vitro of Arabinoxylans(AX-Ⅰ-1) from the Residue of Astragalus Root^†